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2005 Fiscal Year Final Research Report Summary

Elucidation of human folliculogensis by using xenotransplantation of human ovarian tissue

Research Project

Project/Area Number 16591633
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

TERADA Yukihiro  Tohoku University, Hospital, Associate Professor, 病院, 助教授 (10260431)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Takashi  Tohoku University, Graduate school of medicine, Associate Professor, 大学院・医学系研究科, 助教授 (20240666)
Project Period (FY) 2004 – 2005
Keywordshuman ovarian cortex / xenotransplantation / folliculogenesis / matured follicle / human endometrium / endomrtoriosis / NOG mice
Research Abstract

There have been successful reports regarding follicular growth following xenogenic transplantation of the human ovarian cortex into immunodeficient mice. We studied the implantation and development of the human follicle in the graft of non-pathological human ovary following xenogenic transplantation into a immune deficient mice. Human follicles following xenotransplantation into NOD-SCID mice were studied by immunohistochemistry antibodies against the cell proliferation marker and steroidgenic enzymes. Immunohistochmistry suggested that the expression of steroidogenic enzymes in human follicles following xenogenic transplantation into immnunodeficient mice, is similar to that of non-pathological human ovaries. Further, by using NOG mice as a host, we observed development of human graffian follicle following xenotransplantation. The follicular diameter were around 20mm and possess features of dominant follicles. In the series of experiments using NOG mice as a host, we found that ovarian bursa is better place for human ovarian cortex transplantation, as compared kidney capsule or back skin.
As additional series of xenotransplant experiments, we transplanted human endometrium into immunodeficient mice. By hormonal manipulation after xenotransplantation, we succeed to reproduce menstrual changes in transplanted human endometrial tissue. Using this xenotransplantaiotn of human endometrium, we found that a selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis xenografts in immunodeficient mice by decreasing angiogenesis.

  • Research Products

    (22 results)

All 2006 2005 2004 Other

All Journal Article (22 results)

  • [Journal Article] Preface (as an editor Minisymposium : Post ICSI events : Choreography of Fertilization2006

    • Author(s)
      Terada Y.
    • Journal Title

      J Mamm Ova Res. 23

      Pages: 1

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cytoskeletal Dynamics Post-ICSI During Fertilization.2006

    • Author(s)
      Hayasaka S, Terada Y, Morita J, Tachibana M, Shima-Morita J, Kakoi-Yoshimoto T, Nakamura S, Murakami T, Yaegashi N, Okamura K
    • Journal Title

      J Mamm Ova Res. 23

      Pages: 21-26

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cytoskeletal Dynamics Post-ICSI During Fertilization.2006

    • Author(s)
      Hayasaka S, Terada Y, Morita J, Tachibana M, Shima-Morito Y, Kakoi-Yoshimoto T, Nakamura S, Murakami T, Yaegashi N, Okamura K.
    • Journal Title

      J Mamm Ova Res. 23

      Pages: 21-26

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Reproduction of menstrual changes in transplanted human endometrial tissue in immunodeficient mice.2005

    • Author(s)
      Matsuura-Sawada R, Murakami T, Ozawa Y, Nabeshima H, Akahira JI, Sato Y, Koyanagi Y, Ito M, Terada Y, Okamura K.
    • Journal Title

      Hum Reprod 20

      Pages: 1477-84

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Sequential Changes in The Cytoskeletal Dynamics of Microtubules and Microfilaments : Morphological analysis in human spermiogenesis2005

    • Author(s)
      Tachibana M, Terada Y, Murakawa H, Murakami T, Yaegashi N, Okamura K.
    • Journal Title

      Fertile Steril 84

      Pages: 1241-8

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Dynamics of Microtubules and Positioning of Female Pronucleus duringBovine Parthenogenesis.2005

    • Author(s)
      Morito Y, Terada Y, Nakamura S, Morita J, Yoshimoto T, Murakami T, Yaegashi N, Okamura K.
    • Journal Title

      Biol Reprod 73

      Pages: 935-41

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cytoskeletal Dynamics During Mammalian Gametegenesis and Fertilization : Implication For Human Reproduction.2005

    • Author(s)
      Terada Y, Morito Y, Tachibana M, Morita J, Nakamura S, Murakami T, Yaegashi N, Okamura K
    • Journal Title

      Rep Med Biol 4

      Pages: 179-87

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Microtubule organization during rabbit fertilizarion by intracytoplasmic sperm injection with and without sperm centrosome.2005

    • Author(s)
      Morita J, Terada Y, Hosoi Y, Fujinami N, Sugimoto M, Nakamura S, Murakami T, Yaegashi N, Okamura K
    • Journal Title

      Rep Med Biol 4

      Pages: 169-77

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A trial to Restore Defective Human Sperm Centrosomal Function.2005

    • Author(s)
      Nakamura S, Terada Y, Rawe VY, Uehara S, Morita Y, Yoshimoto T, Tachibana M, Murakami T, Yaegashi N, Okamura K
    • Journal Title

      Human Reprod 20

      Pages: 1933-7

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reproduction of menstrual changes in transplanted human endometrial tissue in immunodeficient mice.2005

    • Author(s)
      Matsuura-Sawada R, Murakami T, Ozawa Y, Nabeshima H, Akahira JI, Sato Y, Koyanagi Y, Ito M, Terada Y, Okamura K.
    • Journal Title

      Hum Reprod. 20

      Pages: 1477-1484

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Dynamics of Microtubules and Positioning of Female Pronucleus during Bovine Parthenogenesis.2005

    • Author(s)
      Morito Y, Terada Y, Nakamura S, Morita J, Yoshimoto T, Murakami T, Yaegashi N, Okamura K.
    • Journal Title

      Biol Reprod 73

      Pages: 935-941

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cytoskeletal Dynamics During Mammalian Gametegenesis and Fertilization : Implication for Human Reproduction.2005

    • Author(s)
      Terada Y, Morito Y, Tachibana M, Morita J, Nakamura S, Murakami T, Yaegashi N, Okamura K.
    • Journal Title

      Rep Med Biol 4

      Pages: 179-187

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Microtubule organization during rabbit fertilization by intracytoplasmic sperm injection with and without sperm centrosome.2005

    • Author(s)
      Morita J, Terada Y, Hosoi Y, Fujinami N, Sugimoto M, Nakamura S, Murakami T, Yaegashi N, Okamura K.
    • Journal Title

      Rep Med Biol 4

      Pages: 169-177

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A Trial to Restore Defective Human Sperm Centrosomal Function.2005

    • Author(s)
      Nakamura S, Terada Y, Rawe VY, Uehara S, Morito Y, Yoshimoto T, Tachibana M, Murakami T, Yaegashi N, Okamura K.
    • Journal Title

      Human Reprod 20

      Pages: 1933-1937

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Use of mammalian eggs for assessment of human sperm fynction : molecular and cellular analyses of fertilization by intracytoplasmic sperm injection2004

    • Author(s)
      Terada Y, Nakamura S, Morita J, Tachibana M, Morito Y, Murakami T, Ito K, Yaegashi N, Okamura K.
    • Journal Title

      Am J Repro Immunol 51

      Pages: 290-3

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Centrosomal function assessment in human sperm using heterologus ICSI with rabbit eggs : a new male factor intertility assay2004

    • Author(s)
      Terada Y, Nakamura S, Simerly C, Murakami T, Yaegashi N, Okamura K, Schatten G.
    • Journal Title

      Mol Reprod Dev 67

      Pages: 360-5

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Use of mammalian eggs for assessment of human sperm function : molecular and cellular analyses of fertilization by intracytoplasmic sperm injection2004

    • Author(s)
      Terada Y, Nakamura S, Morita J, Tachibana M, Morito Y, Murakami T, Ito K, Yaegashi N, Okamura K.
    • Journal Title

      Am J Repro Immunol 51

      Pages: 290-293

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Centrosomal function assessment in human sperm using heterologus ICSI with rabbit eggs : a new male factor infertility assay2004

    • Author(s)
      Terada Y, Nakamura S, Simerly C, Hewitson L, Murakami T, Yaegashi N, Okamura K, Schatten G.
    • Journal Title

      Mol Reprod Dev 67

      Pages: 360-365

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A selective cyclooxygenase (COX)-2 inhibitor suppresses the growth of endometriosis xenografts via anti-angiogenic activity in severe combined immunodeficiency mice.

    • Author(s)
      Ozawa Y, Murakami T, Tamura M, Terada Y, Yaegashi N, Okamura K.
    • Journal Title

      Fertile Steri (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Functional assessment of human sperm centrosome : Implication for assisted reproductive technique.

    • Author(s)
      Terada Y, Nakamura S, Tachibana M, Shima Y, Morita J, Hayasaka S, Murakami T, Yaegashi N, Okamura K
    • Journal Title

      Curr Top Dev Biol (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A selective cyclooxygenase (COX)-2 inhibitor suppresses the growth of endometriosis xenografts via anti-angiogenic activity in severe combined immunodeficiency mice.

    • Author(s)
      Ozawa Y, Murakami T, Tamura M, Terada Y, Yaegashi N, Okamura K.
    • Journal Title

      Fertile Steri (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Functional assessment of human sperm centrosome : Implication for assisted reproductive technique.

    • Author(s)
      Terada Y, Nakamura S, Tachibana M, Shima Y, Morita J, Hayasaka S, Murakami T, Yaegashi N, Okamura K
    • Journal Title

      Curr Top Dev Biol (in press)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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