2005 Fiscal Year Final Research Report Summary
Growth Inhibition of Head and Neck Squamous Carcinoma Cells by Small Interfering RNAs Targeting Cyclin D1
| Project/Area Number |
16591694
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
ORIDATE Nobuhiko Hokkaido Univ., Hokkaido University Hospital, Inst., 病院, 講師 (90312355)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Head and neck squamous cell carcinomas / cyclin D1 / small interfering RNA / inhibition of the cancer cell growth |
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| Research Abstract |
Head and neck squamous cell carcinomas (HNSCCs) exhibit an increased expression of the cell-cycle regulator cyclin D1 (CCND1). It stimulates cell cycle progression and transform squamous epithelial cells. We used small interfering RNAs (siRNAs) to silence the expression of CCND1 in HNSCC UMSCC22B cells and analyzed the effects of reduced levels of these proteins on colony formation. Transfection of CCND1 siRNAs decreased the levels of their targeted proteins and inhibited cell growth. Combination of this siRNAs and cisplatin showed more than additive inhibition of colony formation. These findings demonstrate that siRNA silencing of CCND1 leads to inhibition of the growth of HNSCC cells and suggest that CCND1 siRNAs alone or combined with conventional cytotoxic agents may be useful for therapy of HNSCCs.
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