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2006 Fiscal Year Final Research Report Summary

Research on regulatory mechanism of ion transport in the endolymphatic sac and its distubance

Research Project

Project/Area Number 16591711
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKagawa University

Principal Investigator

MORI Nozomu  Kagawa University, Faculty of Medicine, Professor, 医学部, 教授 (90124883)

Co-Investigator(Kenkyū-buntansha) MIYASHITA Takenori  Kagawa University, Faculty of Medicine, Assistant, 医学部, 助手 (60363214)
Project Period (FY) 2004 – 2006
Keywordsendolymphatic sac / ion transport / patch clamp technique / RT-PCR / in situ hydridization / regulatory factors of ion transport
Research Abstract

(Microfluorometrical study on ion channel in the ES)
The dynamic properties of sodium transport in the endolymphatic sac (ES) were investigated using SFBI/AM fluorescence measurement. The intracellular Na^+ concentration was measured in freshly dissected ES sheet from the intermediate portion of the ES of the guinea pig. The results showed the strong relationship between Na^+, K^+ATPase activity and mitochondrial staining, suggesting that mitochondria-rich ES epithelial cells (Type 1 cells, ca. 30% of ES cells) endowed with high Na+ permeability and Na^+, K^+-ATPase activity potentially contribute to sodium transport of the ES.
(Molecular biological study of ion channels and ion transporters)
The presence of Na-K-2Clcotransporter(NKCC) and P_<2Y> receptor and their subtypes in the ES of the rat was examined by molecular biological techniques. RT-PCR showed the presence of two subtypes of NKCC (NKCC1 and NKCC2) and four subtypes of P<2Y> receptor (P_2Y_1,P_2Y_2,P_2Y_4,P_2Y_6). The presence of NKCC2 (peculiar to the kidney) in the ES strongly suggests the absorptive function of the ES. RT-PCR confirmed the presence of P2Y receptors suggested by our previous electrophysiological study. We are now examining the localization of NKCC and P_<2Y> receptor in the ES.

  • Research Products

    (6 results)

All 2007 2005

All Journal Article (6 results)

  • [Journal Article] Large Na^+ influx and high Na^+, K^+-ATPase activity in mitochondria -rich epithelial cells of the inner ear endolymphatic sac2007

    • Author(s)
      Miyashita Takenori
    • Journal Title

      Eur J Physiol 453

      Pages: 905-913

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Large Na^+ influx and high Na^+, K^+-ATPase activity in mitochondria-rich epithelial cells of the inner ear endolymphatic sac.2007

    • Author(s)
      Miyashita Takenori
    • Journal Title

      Eur.J.Physiol. 453

      Pages: 905-913

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cation transport in epithelial cells of the endolymphatic sac2005

    • Author(s)
      Mori Nozomu
    • Journal Title

      Proceedings of 5^<th> International Symposium : Meniere's Disease & Inner Ear Homeostasis Disorders ISBN0-9776204-0-9

      Pages: 52-54

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mitochondria-rich epithelial cells play an important role in Na^+ transport of the endolymphatic sac2005

    • Author(s)
      Miyashita Takenori
    • Journal Title

      Proceedings of 5^<th> International Symposium : Meniere's Disease & Inner Ear Homeostasis Disorders ISBN0-9776204-0-9

      Pages: 258-260

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cation transport in epithelial cells of the endolymphatic sac.2005

    • Author(s)
      Mori Nozomu
    • Journal Title

      Proceedings of 5^<th> International Symposium : Meniere's Disease & Inner Ear Homeostasis Disorders ISBN0-9776204-0-9

      Pages: 52-54

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mitochondria-rich epithelial cells play an important role in Na+ transport of the endolymphatic sac2005

    • Author(s)
      Miyashita Takenori
    • Journal Title

      Proceedings of 5^<th> International Symposium : Meniere's Disease & Inner Ear Homestasis Disorders ISBN0-9776204-0-9

      Pages: 258-260

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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