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2005 Fiscal Year Final Research Report Summary

Study of effects of antiglaucoma eye drops on glaucomatous optic neuropathy and ocular serface.

Research Project

Project/Area Number 16591738
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionHirosaki University

Principal Investigator

OHGURO Ikuyo  Hirosaki University, School of Medicine, lecturer, 医学部, 講師 (90305235)

Co-Investigator(Kenkyū-buntansha) OHGURO Hiroshi  Hirosaki University, School of Medicine, associate professor, 医学部, 助教授 (30203748)
NAKAZAWA Mitsuru  Hirosaki University, School of Medicine, professor, 医学部, 教授 (80180272)
MAMIYA Kazuhisa  Hirosaki University, University Hospital, Instructor, 医学部附属病院, 助手 (60344610)
Project Period (FY) 2004 – 2005
Keywordsantiglaucoma eye drops / neuroprotection / ocular surface / NMDA / α-adrenergic receptor / extracellular matrix / MMP / TIMP
Research Abstract

Purpose : To study the effects of antiglaucoma eye drops on N-methyl-D-aspartate (NMDA)-induced retinal damage and ocular surface
Methods : Several antiglaucoma eye drops, β-blockers, α/β-blockers, an α_1-blocker, and α_2-agonist, and a prostaglandin derivative, were topically administrated to NMDA-treated rat eyes or non-treated rat eye, and the retinal thickness, the number of retrograde-labeled retinal ganglion cells (RGCs), the results of a cDNA microarray analysis, and the levels of matrix metalloprotease (MMP) and tissue inhibitor of matrix protease (TIMP) which regulate the metabolism of the extracellular matrix (ECM) were studied.
Results : Intravitreal administration of NMDA caused a significant decrease in the thickness of the retinal layers and induced upregulation of glial fibrillary protein (GFAP). Topical administration of β-blockers and a prostaglandin derivative showed almost no significant effects on retinal thickness, the number of RGCs, or expression of GFAP. In contrast, the α/β-blockers, the α_1-blocker, and the α_2-agonist showed preservation effects on retinal thickness and the the number of RGCs, and marked suppression of NMDA-induced upregulation of GFAP. Among 1101 genes related to cellular regulatory mechanisms, the expression of two genes, both for insulin-like growth factors, IGF-1 and ErbB3,was altered upon administration of the α/β-blockers, the α_1-blocker, and the α_2-agonist. In addition, topical administration of the α/β-blockers, the α_1-blocker, and the prostaglandin derivative induced upregulation of MMP and downregulation of TIMP of conjunctiva.
Conclusion : Our present study suggests that modulations of the α-adrenergic receptor, α_1-blocking, and α_2-stimulation, by antiglaucoma eye drops may cause beneficial effects on NMDA-induced retinal damage in the rat. Our results also suggests that the α/β-blockers, the α_1-blocker, and the prostaglandin derivative induce degradation of ECM, affcting filter surgery.

  • Research Products

    (9 results)

All 2005 2004

All Journal Article (8 results) Book (1 results)

  • [Journal Article] Optic disc characteristics assessed by evaluation of clinical optic disc photographs in glaucoma patients.2005

    • Author(s)
      Ohguro I, Ohguro H, et al.
    • Journal Title

      Hirosaki Med. J. 57

      Pages: 27-34

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Study of effects of antiglaucoma eye drops on N-Methyl-D-Aspartate-induced retinal damage.2005

    • Author(s)
      Metoki T, Ohguro I, et al.
    • Journal Title

      Jpn J Ophthalmol 49

      Pages: 453-461

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 緑内障性視神経障害における眼圧非依存因子の検討-血小板凝集能との係わり-2005

    • Author(s)
      大黒幾代, 大黒 浩, 他
    • Journal Title

      あたらしい眼科 22

      Pages: 669-672

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 抗緑内障点眼薬のFDTによる神経保護効果の検討2005

    • Author(s)
      竹内侯雄, 大黒幾代, 他
    • Journal Title

      眼科紀要 56

      Pages: 134-137

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Optic disc characteristics assessed by evaluation of clinical optic disc photographs in glaucoma patients.2005

    • Author(s)
      Ohguro I, Ohguro H, et al.
    • Journal Title

      Hirosaki Med J 57

      Pages: 27-34

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of matrix metalloproteinase-3 gene transfer by electroporation in glaucoma filter surgery.2004

    • Author(s)
      Mamiya K, Ohguro I, et al.
    • Journal Title

      Exp Eye Res 79

      Pages: 405-410

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 点眼薬による眼表面の反応 抗緑内障点眼薬の眼表面への影響と神経保護の検討2004

    • Author(s)
      大黒 浩, 大黒幾代, 他
    • Journal Title

      眼薬理 18

      Pages: 69-73

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of matrix metalloprotease-3 gene transfer by electroporation in glaucoma surgery.2004

    • Author(s)
      Mamiya K, Ohguro I, et al.
    • Journal Title

      Exp Eye Res 79

      Pages: 405-410

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Frontiers in Glaucoma2005

    • Author(s)
      大黒 浩, 大黒幾代, 他
    • Total Pages
      7
    • Publisher
      メディカルレビュー社
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2007-12-13  

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