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2005 Fiscal Year Final Research Report Summary

Transplantation of enriched photoreceptor precursor cells into degenerated retina and conditioning of host retina.

Research Project

Project/Area Number 16591748
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKyoto University

Principal Investigator

AKIMOTO Masayuki  Kyoto University, Medicine, Assistant Professor, 医学研究科, 助手 (90303453)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Masayo  Kyoto University, Medicine, Associate Professor, 医学研究科, 助教授 (80252443)
MANDAI Michiko  Kyoto University, Medicine, Assistant Professor, 医学研究科, 助手 (80263086)
Project Period (FY) 2004 – 2005
Keywordsphotoreceptor / regenerative medicine / transgenic mice / retinal transplantation / in situ hybridization
Research Abstract

We previously generated transgenic mice (NrlGFP mice) in which the photoreceptor and its precursor cells are labeled by GFP. We tested the usefulness of this transgenic mice for retinal transplantation study. In first, we investigated the effect of enriched photoreceptor precursor cells into degenerated retina histologically and physiologically. We tested if the treatment with chondroitinase ABC (ChABC), an enzyme that degrades chondroitin sulfate proteoglycans, can promote synapse formation between graft and host neurons following retinal transplantation. We also investigated the gene profile of photoreceptor like cells transdifferentiated from iris pigment epithelial (IPE) cells assisted by gene transfer.
Up to four weeks after transplantation, almost all the grafted GFP+ photoreceptor cells were widely distributed at the outer margin of the host retina where the photoreceptor layer was located originally. In the Nrl/ChABC group,33.6% of the GFP+ photoreceptors elaborated neurites horizontally or vertically, and 4.6% elaborated neurites toward the retina. These neurites extended over the glial seal at the graft-host interface, and established synaptic contacts with neurons in the host retina as determined by confocal microscopy and three-dimensional analysis. Although 30.7% cells (P=0.68) elaborated neurites in the Nrl group, only 1.2% cells (P<0.05) projected neurites towards the host tissue and synaptic contacts were rare.
Microarray analyses provided lists of photoreceptor specific and IPE specific genes. We are currently testing those genes as markers for effectiveness of transdifferetiation.

  • Research Products

    (4 results)

All 2005

All Journal Article (4 results)

  • [Journal Article] A new PCR-based approach for the preparation of RNA probe2005

    • Author(s)
      Suzuki T et al.
    • Journal Title

      Journal of Biochemical and Biophysical Methods 62・3

      Pages: 251-258

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 網膜視細胞の分化誘導と移植医療の可能性2005

    • Author(s)
      秋元 正行
    • Journal Title

      再生医学 4

      Pages: 71-76

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A new PCR-based approach for the preparation of RNA probe2005

    • Author(s)
      Suzuki T et al.
    • Journal Title

      Journal of Biochemical and Biophysical Methods 62-3

      Pages: 251-258

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regeneration of retinal photoreceptors and retinal transplantation : review2005

    • Author(s)
      Akimoto M.
    • Journal Title

      Regenerative Medicine 4

      Pages: 71-76

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

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