2005 Fiscal Year Final Research Report Summary
"Good basement membrane" will inhibit diabetic retinopathy
| Project/Area Number |
16591755
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Okayama University |
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Principal Investigator |
KUSACHI Shozo Okayama University, Medical School, professor, 医学部, 教授 (30214943)
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| Co-Investigator(Kenkyū-buntansha) |
NINOMIYA Yoshifumi Okayama University, Graduate School of Medicine, Dentistry Phamaceutical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (70126241)
HIROHATA Satoshi Okayama University, Graduate School of Medicine, Dentistry Phamaceutical Sciences, Assistant, 大学院・医歯薬学総合研究科, 助手 (90332791)
SHIRAGA Fumio Kagawa University, Medical School, Professor, 医学部, 教授 (50187530)
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| Project Period (FY) |
2004 – 2005
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| Keywords | extracellular matrix / laminin / diabetic retinopathy / basement membrane |
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| Research Abstract |
Diabetic retinopathy is characterized as increased angiogenesis as well as leaky vessels resulted in abnormal bleeding. The aim of our study was to define the role of basement membrane for diabetic retinopathy. We hypothesized that a breakdown or an abnormal organization of basement membrane was the initial step of the pathological change in the diabetic retinopathy that leads to the vulnerability of the vessels.
We focused on the two components of basement membrane, laminin and type IV collagen.
1. Leaky vessels in diabetic retinopathy
Diabetic rat model was made by administration of streptozotocin (STZ) (180mg/kg, i.p.). The blood glucose level was measured. All the rats demonstrated continued high blood glucose level (>250mg/dl). We examined the retina of the diabetic rat by using Evans blue staining. Despite the diabetic state, our rats did not show particular evidence of leaky vessels.
2. Distribution of basement membrane components in diabetic retinopathy
We examined the distribution of the major components of basement membrane, laminin and type IV collagen. We used specific antibody against from α1 to α6 chains of type IV collagen and α4 and α5 chain of laminin for the immunofluorescent staining analysis.
In the vascular basement membrane of the retina, α1 and α2 chains of type IV collagen was observed, while internal limiting membrane (ILM) showed positive signals for α5 and α6 chains of collagen IV as well as α1 and α2 chains of collagen IV.
3. The change of basement membrane components and the diabetic retinopathy
We concluded that the establishment of proper model for diabetic retinopathy is crucial. The transgenic animal of basement membrane may be of interest.
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