2005 Fiscal Year Final Research Report Summary
The development for new inhibitory methods for corneal graft rejection by using NKT cells
| Project/Area Number |
16591757
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
SONODA Koh-Hei Kyushu University, Graduate School of Medical Science, Assistant Professor, 大学院・医学研究院, 助手 (10294943)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Hiroki Saga University, Faculty of Medicine, Professor, 医学部・分子生命科学講座, 教授 (40260715)
EGASHIRA Kensuke Kyushu University, Graduate School of Medical Science, Assosiate Professor, 大学院・医学研究院, 助教授 (60260379)
HATA Yasuaki Kyushu University, Graduate School of Medical Science, Assistant Professor, 大学院・医学研究院, 講師 (90346776)
YOSHIDA Shigeo Kyushu University, Graduate School of Medical Science, Assistant Professor, 大学院・医学研究院, 助手 (50363370)
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| Project Period (FY) |
2004 – 2005
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| Keywords | corneal transplantation / NKT cell / cytokine / tolerance / DNA chip / gene transfer / immune therapy |
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| Research Abstract |
Analysis in murine corneal allo graft (in vivo)
NKT cells were enriched from spleen and peripheral blood of grafted mice seven days later. NKT cells were enriched by magnetic beads. We analyzed cytokine and chemokine produced from these NKT cells. NKT cells were cultured with IL-2 and IL-15 and stimulated by aGalCer. We found NKT cells produced IL-10.
The expansion of human NKT cells (in vitro)
NKT cells were enriched from either long term accepted patients or healthy volunteers. We enriched NKT cells with more than 90% purity by magnetic beads. As we did in mice, NKT cells were cultured and expanded by aGalCer with IL-2 and IL-15.
The screening of cytokine and chemokines produced by NKT cells
We screened specific cytokines and chemokines in graft-accepted patients. We found NKT cells from accepted patients predominantly produced IL-10. In contrast, they reduced IFNg, TNFa and RANTES production.
Gene transfer of IL-10 into cultured NKT cells
We succeeded to transfer IL-10 gene into cultured human NKT cells, and confirmed IL-10 production in vitro.
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