2005 Fiscal Year Final Research Report Summary
Expression of TRAF family proteins and TRAF-mediated signal transduction in the cornea
| Project/Area Number |
16591772
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Nihon University |
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Principal Investigator |
IWATA Mitsuhiro Nihon University, 医学部, Assistant Professor (50193751)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Yoshihiro Nihon University School of Medicine, 医学部, Assistant (80206549)
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| Project Period (FY) |
2004 – 2005
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| Keywords | cornea / TRAF / CD40 / signal transduction / protein tyrosine kinase / GM-CSF / IL-8 |
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| Research Abstract |
1. We examined the expression of TRAF family proteins in normal human cornea and cultured human corneal cells by immunostaining technique.
1) Expressions of TRAF1, 2, and 4 were observed in all corneal component cells. Expressions of TRAF5 and 6 were observed constantly in corneal epithelium, whereas in corneal stroma and endothelium, either expression differed among donors. TRAF3 was never expressed in all corneal cells. Intracellular expressions of each TRAF were different depending upon corneal cell types.
2) In cultured human corneal epithelial (HCE) and stromal (HCS) cells, all TRAFs except for TRAF3 were expressed constitutively. The pattern of intracellular expression of each TRAF was different from those in normal human cornea. TRAF4 was expressed in both the nucleus and cytoplasm, and TRAF1, 5, and 6 were expressed in the cytoplasm, irrespective of corneal cell types. The biological significance of these findings should be investigated in the future study.
2. We investigated the significance of protein tyrosine kinase (PTK)-dependent signal transduction in CD40-mediated production of proinflammatory cytokines in the corneal cells. CD40 ligation with anti-CD40 monoclonal antibody induced production of GM-CSF and IL-8 by cultured HCE and HCS cells through a PTK-dependent pathway. The relationship between PTK-dependent and TRAF-dependent signaling pathways in CD40-mediated production of GM-CSF and IL-8 by the corneal cells remains to be elucidated.
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