2005 Fiscal Year Final Research Report Summary
Predicting Onset of Secondary Multiple Organ Failure by Profiling of Cytokine Gene Expression
| Project/Area Number |
16591806
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Chiba University |
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Principal Investigator |
SHIGA Hidetoshi Chiba University, Hospital, Assistant Professor, 医学部附属病院, 講師 (20282478)
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| Co-Investigator(Kenkyū-buntansha) |
HIRASAWA Hiroyuki Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (80114320)
ODA Shigeto Chiba University, Graduate School of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (90204205)
MATSUDA Kenichi Chiba University, Hospital, Assistant Professor, 医学部附属病院, 講師 (60282480)
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| Project Period (FY) |
2004 – 2005
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| Keywords | SIRS / gene expression / cytokine / IL-1 / IL-6 / TNF-alpha / IL-10 / RT-PCR |
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| Research Abstract |
Twenty ICU patients with sepsis were enrolled to the study. mRNA was isolated from the peripheral blood on admission and on day 2 in ICU. Real-time reverse transcriptase PCR was used to quantify mRNA expression of TNF-alpha, IL-1beta, IL-1 receptor antagonist, IL-6,IL-8,IL-10 and IFN-gamma. The mRNA expression levels were assessed by percent changes on day 2 compared to that on admission. Serum levels of cytokines on admission and monocyte HLA-DR expression on day 3 were also measured.
Between survivors and non-survivors, there was no difference in mRNA expression levels of all mediators except for IL-10. IL-10 mRNA level on day 2 compared to that on admission was significantly up-regulated in non-survivors.
When the survival rate was compared between the mRNA expression level up-regulated group and the down-regulated group, it was significantly higher in IL-10 up-regulated group.
Up-regulation of IL-10 mRNA expression significantly associated with the diminished HLA-DR expression on monocytes (r=0.78,p<0.001). To the contrary, serum IL-10 level on admission did not correlate with HLA-DR expression.
Up-regulated IL-10 mRNA expression in peripheral leukocytes could be a useful predictor of not only the poor outcome, but also diminishing HLA-DR expression in septic patients. The potential usefulness of IL-10 mRNA expression measurement for the management of septic patients should be further evaluated.
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