2005 Fiscal Year Final Research Report Summary
Developments of free radical monitoring and treatment in neuro-emergency diseases
| Project/Area Number |
16591815
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Showa University |
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Principal Investigator |
ARUGA Tohru Showa University, School of Medicine, Professor, 医学部, 教授 (40266086)
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| Co-Investigator(Kenkyū-buntansha) |
DOHI Kenji Showa University, School of Medicine, Assistant, 医学部, 助手 (20301509)
SATOH Kazue Showa University, School of Medicine, Professor, 医学部, 教授 (90053941)
SHIODA Seiji Showa University, School of Medicine, Professor, 医学部, 教授 (80102375)
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| Project Period (FY) |
2004 – 2005
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| Keywords | free radical / activated oxygen. / monitoring / brain injury / alkoxy radical / lipid peroxidation / human / brain hypothermia |
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| Research Abstract |
Recent experimental studies have demonstrated that oxygen free radicals have important roles of various neuronal conditions. Especially, Neuroemergency diseases such as neurotrauma, stroke and ischemia after cardiac arrest are strongly related with oxidative stress. However, there is no study proved the existence of free radicals in human because of difficulty in measurement. In this study we developed free radical (alkoxyl radical) monitoring in neuroemergency patients using extra vivo electron spin resonance (ESR) spectroscopy.
Blood samples were collected from the catheter of the internal jugular bulb. The control samples were also collected from healthy volunteers. The alkoxyl radical level was measured extra vivo by ESR spectrometry using 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) as a spin trap, which was obtained from Dojin Chemical (Tokyo, Japan). Electron spin response detection of the spin adduct was performed at room temperature using a JESREIX X-band spectrometer (JEOL, Tokyo, Japan).
Alkoxyl radical levels of all patients were elevated in comparison with control. Alkoxyl radical level was suppressed after administration of edaravone (30mg i.v). Brain hypothermia treatment suppressed alkoxyl radical in the injured brain while conversely causing systemic oxidative stress formation. Alkoxyl radical levels in patients treated with hyperbaric oxygen therapy (HBO) were elevated within treatment.
Conclusion :
We proved the elevation of intravenous free radical levels in neuroemergency patients. This method is useful to monitor the free radical level immediately at bedside and it will enable to detect the oxidative stress and the severity of brain damage.
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