2005 Fiscal Year Final Research Report Summary
The development of assay method for endocannabinoids and their prostaglandin derivatives in medical samples.
| Project/Area Number |
16591816
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | The Jikei University School of Medicine |
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Principal Investigator |
OBATA Toru The Jikei University School of Medicine, Associate Professor, 医学部, 助教授 (80119776)
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| Project Period (FY) |
2004 – 2005
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| Keywords | endocannabinoids / anandamide / 2-AG / GCMS / LCMS / Isotope dilution method / septic shock |
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| Research Abstract |
The endocannabinoids assay with using isotope dilution method by GC/MS and LC/MS/MS are developed for medical samples. These assay system were used for endocannabinoids assay in patients plasma which were collected during the haemodialysis treatment in endotoxic shock. The results indicate the evaluation of haemodialysis treatment for endotoxic shock.
Another theme of this study for the prostaglandin derivative assay are tried for medical samples. Although the successful to detect authetic standard of prostaglandin delivative by LC/MS/MS, we could not assay them in medical samples acculately for their low and/or poor concentration. We have to make a proper internal standard, such as deuterium labeled compound, of these prostaglandin delivatives for acculate assay.
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