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2005 Fiscal Year Final Research Report Summary

The role of Il-18 In Inducing or preventing apoptosis in neutrophils during acute endotoxemia

Research Project

Project/Area Number 16591818
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Emergency medicine
Research InstitutionHyogo College of Medicine

Principal Investigator

KOTANI Joji  Hyogo College of Medicine, Faculty of Medicine, Research Associate, 医学部, 助手 (80360270)

Co-Investigator(Kenkyū-buntansha) OHYA Munehiko  Hyogo College of Medicine, Faculty of Medicine, Research Associate, 医学部, 助手 (40309480)
OKAMURA Haruki  Hyogo College of Medicine, Faculty of Medicine, Professor, 医学部, 教授 (60111043)
MARUKAWA Seishiro  Hyogo College of Medicine, Faculty of Medicine, Professor, 医学部, 教授 (00030883)
Project Period (FY) 2004 – 2005
KeywordsIL-18 / neutrophil / bone marrow cells / endotoxin / popy unsaturated fatty acids / mouse / rat / tumor necrosis factor
Research Abstract

1.The role of TNR receptors in bone marrow cell apoptosis during endotoxin-induced systemic inflammatory state. Apoptosis of myeloid cells in the bone marrow during endotoxemia is minimized as these cells undergo differentiation, which in turn, may be due to the attenuation of the pro-apoptotic effect of TNFR-p55 shown herein to occur with myeloid differentiation. In contrast, TNFR-p75 appears to play a minimal role in apoptosis induction in Grl(-) myeloid cells during endotoxemia. One explanation for a decrease in bone marrow cell numbers during endotoxemia may be via induction of apoptosis in immature myeloid cells.
2.The role of IL-18 in bone marrow cell apoptosis during endotoxin-induced systemic inflammatory state. It has been demonstrated that IL-18 plays a anti-apoptotic role in mature and immature myeloid cells in the bone marrow during endotoxin-induced systemic inflammatory state.
3.The role of IL-18 in both nitric oxide production and pancreatic damage during acute pancreatiti … More s. IL-18 appears to protect the pancreas during early caerulein-induced AP in mice, probably through induction of NO release from an iNOS source. IL-18 may be a target for new AP therapeutics.
4.Establishment of the technique of total parenteral nutrition (TPN) in rats. We have developed the techniques to insert and fix the silicon tube into the jugular vein, transfuse TPN solution for three days safely without restriction in Wister rats.
5.Apoptosis induction in the bone marrow cells during endotoxin-induced systemic inflammatory state in rats. We have confirmed that apoptosis is induced in both immature and mature bone marrow cells after intra-peritoneal injection of endotoxin like apoptosis induction in the bone marrow cells in mouse acute endotoxemia model.
6.The influence of pretreatment with TPN with n-6 and n-3 poly unsaturated fatty acids (PUFAs) on apoptosis in bone marrow cells and peripheral neutrophils during endotoxin-induced systemic inflammatory state. The HPLC analysis revealed that three days TPN could change the cell wall components of spleen cells and red blood cells. Furthermore, we have found that pretreatment with TPN with n-3 PUFAs appears to increase apoptotic cell rate in the immature and mature bone marrow cells and peripheral neutrophils compared to TPN with n-6 PUFAs although the mechanisms have not been elucidated.
7.The role of IL-18 in human neutrophils apotosis. IL-18, like LPS, inhibits PMN apoptosis by activating PI3-kinase and ERK pathways. However, PI3-kinase plays more important role(s) in IL-18-induced inhibition of apoptosis than in LPS-induced apoptosis inhibition.
8.The influence of short chain fatty acids (SCFAs). Butyrate activates caspase cascade and induces apoptosis via signal transduction(s) other than GPR and MAPKs. It is most likely that butyrate modulate apoptosis via HDAC inhibition. Less

  • Research Products

    (12 results)

All 2006 2005

All Journal Article (12 results)

  • [Journal Article] Tumor necrosis factor receptor regulation of bone marrow cell apoptosis during endotoxin-induced systemic inflammation2006

    • Author(s)
      Joji Kotani
    • Journal Title

      Shock 25(5)

      Pages: 464-471

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 腸管上皮細胞におけるToll-likereceptorsの役割2006

    • Author(s)
      小谷穣治
    • Journal Title

      日本外科代謝・栄養 39・4

      Pages: 165-177

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 侵襲下における骨髄細胞アポトーシスとTNFレセプターの役割-好中球への成熟に伴う変化-2006

    • Author(s)
      小谷穣治
    • Journal Title

      侵襲と免疫 15・1

      Pages: 37-40

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Tumor necrosis factor receptor regulation of bone marrow cell apoptosis during endotoxin-induced systemic inflammation.2006

    • Author(s)
      Joji Kotani, Nicholas J.Avallon, Edward Lin, Masahiro Goshima, Stephen F.Lowry, Steve E.Calvano.
    • Journal Title

      Shock (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 侵襲下における骨髄細胞アポトーシスとTNFレセプターの役割.-好中球への成熟に伴う変化-2006

    • Author(s)
      小谷穣治.
    • Journal Title

      侵襲と免疫 15(1)

      Pages: 37-40

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 重症感染症における臓器障害の発生機序2005

    • Author(s)
      小谷穣治
    • Journal Title

      日本腹部救急医学会雑誌 25・5

      Pages: 713-720

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Apoptosis And Bcl-2 Protein Family Expression In Neutrophils During Experimental Human Endotoxemia And Clinical2005

    • Author(s)
      Joji Kotani
    • Journal Title

      Shock 23・suppl

      Pages: 58-59

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Role Of Interleukin 18 In Nitric Oxide Production And Pancreatic Damage During Acute Pancreatitis2005

    • Author(s)
      Naoko Ueno
    • Journal Title

      Shock 24・6

      Pages: 564-570

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The mechanisms of organ dysfunction during severe infection.2005

    • Author(s)
      Joji Kotani, Jun-ichi Hirata, Masahiro Goshima, MD, PhD, Mariko Yamada, Munenori Matsudaira, Munehiko Ohya, Manabu Kirita, Kazumasa Yoshinaga, Seishiro Marukawa.
    • Journal Title

      日本腹部救急医学会雑誌 25(5)

      Pages: 718-720

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The roles of toll-like receptors in gut epithelium.2005

    • Author(s)
      Joji Kotani, Masahiro Goshima, Michiko Aoyama, Manabu Kirita, Makoto Usami, Seishiro Marukawa.
    • Journal Title

      JSME 39(4)

      Pages: 165-177

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] APOPTOSIS AND BCL-2 PROTEIN FAMILY EXPRESSION IN NEUTROPHILS DURING EXPERIMENTAL HUMAN ENDOTOXEMIA AND CLINICAL SEPSIS.2005

    • Author(s)
      J Kotani*, E Lin*, N Avallone*, M Goshima*, S Calvano, S Lowry.
    • Journal Title

      Shock 23, Supple 3

      Pages: 58-59

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] ROLE OF INTERLEUKIN 18 IN NITRIC OXIDE PRODUCTION AND PANCREATIC DAMAGE DURING ACUTE PANCREATITIS2005

    • Author(s)
      Naoko Ueno, Shin-ichiro Kashiwamura, Haruyasu Ueda, Haruki Okamura, Noriko M. Tsuji, Katsushi Hosohara, Joji Kotanii, Seishiro Marukawa.
    • Journal Title

      Shock 24(6)

      Pages: 564-570

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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