2005 Fiscal Year Final Research Report Summary
Effects of Growth Factors and Carcinogens on the Development of Odontogenic Tumors
| Project/Area Number |
16591849
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Aichi-Gakuin University |
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Principal Investigator |
KAMEYAMA Yoichiro Aichi-gakuin University, School of Dentistry, Department of Pathology, Professor, 歯学部, 教授 (70113066)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Hatsuhiko Aichi-gakuin University, School of Dentistry, Department of Pathology, Associate Professor, 歯学部, 助教授 (30175591)
SATO Emiko Aichi-gakuin University, School of Dentistry, Department of Pathology, Lecturer, 歯学部, 講師 (60111001)
KUBO Katsutoshi Aichi-gakuin University, School of Dentistry, Department of Pathology, Lecturer, 歯学部, 講師 (60329604)
SUGITA Yoshihiko Aichi-gakuin University, School of Dentistry, Department of Pathology, Assistant, 歯学部, 助手 (20367618)
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| Project Period (FY) |
2004 – 2005
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| Keywords | animal / jaw bone / tooth germ / organ culture / heterocyclic amine / growth factor |
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| Research Abstract |
In the present study, the effects of both growth factor TGF-α and carcinogen Trp-P-2 on the development of organ-cultured ddY mouse tooth-germ transplanted into the subcutaneous tissue of nude mice were investigated historically and immnohistochemically (PCNA) in the following 5 Groups of mice : Group A (Control), Group B (DMSO), Group C (TGF-α), Group D (Trp-P-2) and Group E (TGF-α+Trp-P-2). Historically, in Groups C,D and E, a marked proliferation of odontogenic epithelial cells was observed. In Groups D and E, keratinization of the odontogenic epithelial cells was found, but it was not observed in Group C. Moreover, in Group E, a cyst-like space was observed in the area of keratinized odontogenic epithelial cells. Immunohistochemically (PCNA), in Groups C,D and E, the positivities of the odontogenic epithelial cells were significantly higher than in Groups A and B. The results of the present study suggest that proliferation and keratinization of odontogenic epitherial cells of transplanted organ-cultured tooth-germs in mice are promoted by growth factor TGF-α and carcinogen Trp-P-2.
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