2007 Fiscal Year Final Research Report Summary
ROLE OF NEUROPEPTIDEY IN THE SYMPATHETIC NERVE REGULATION OF BONE METABOLISM
| Project/Area Number |
16591876
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
ARAI Michitsugu Aichi Gakuin University, SCH. DENT., ASSISTANT PROF. (20097538)
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| Co-Investigator(Kenkyū-buntansha) |
TOGARI Akifumi AICHI-GAKUIN UNIVERSTTY, SCH. DENT., PROFESSOR (80126325)
HIRUKAWA Koji AICHI-GAKUIN UNIVERSTTY, SCH. DENT., RESEARCH ASSOCIATE (60340147)
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| Project Period (FY) |
2004 – 2007
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| Keywords | neuropeptide Y / isoprenaline / osteoclastogenesis / bone marrow cell / sympathetic nerve / bone metabolism / cAMP / Y1 receptor |
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| Research Abstract |
In this project, we demonstrated a physiological role of neuropeptide Y (NPY), a co-transmitter with noradrenaline in peripheral sympathetic nerve fibers, in the nerve regulation of bone metabolism. The effect of NPY on the osteoclastogenesis in mouse bone marrow cell cultures treated with isoprenaline, a β-adrenergic receptor (β-AR) agonist, was examined. The mouse bone marrow cells constitutively expressed mRNAs for the NPY-Y1 receptor and β2-AR. NPY inhibited the formation of osteoclast-like cells induced by isoprenaline but not that by 1α, 25-dihydroxyvitamin D_3 (1a, 25(OH)_2D_3) or soluble receptor activator of nuclear factor-B_K ligand (RANKL); and it suppressed the production of RANKL and cyclic AMP (cAMP) increased by isoprenaline but not those increased by 1α, 25(OH)_2D_3. NPY also inhibited osteoclastogenesis induced by forskolin, an activator of adenylate cyclase; however, it did not inhibit that induced by exogenously supplied dibutyryl cAMP, a cell-permeable cAMP analog that activates cAMP-dependent protein kinase. These results demonstrate that NPY inhibited the isoprenaline-induced osteoclastogenesis by blocking the agonist-elicited increases in the production of cAMP and RANKL in mouse bone marrow cells, suggesting an interaction between NPY and β-AR agonist in bone resorption.
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Research Products
(25 results)
