Antitumor effect of gene transfection and DNA vacccine of beta-defensins

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 16591894
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: ABIKO Yoshihiro Health Sciences University of Hokkaido, Institute of Personalized Medical Science, Professor (90260819)
• Co-Investigator(Kenkyū-buntansha): KAKU Tohru Health Sciences University of Hokkaido, School of Dentistry, Professor (60133253) ; ARAKAWA Toshiya Health Sciences University of Hokkaido, School of Dentistry, Assistant Professor (40306254) ; NAGAYASU Hiroki Health Sciences University of Hokkaido, Institute of Personalized Medical Science, Associate Professor (90265075) ; OKUMURA Kazuhiko Health Sciences University of Hokkaido, School of Dentistry, Assistant Professor (60194510)
• Project Period (FY): 2004 – 2007
• Keywords: Beta-defensin / Antitumor / Gene therapy / DNA vaccine / Squamous cell carcinoma / Lymphoma

Research Abstract

The present study examined whether increased expression of beta-defensins by the gene transfection affected antitumor of squamous cell carcinoma (SCC), and whether DNA vaccine with beta-defensins affected antitumor of lymphoma. In order to observe the antitumor effect of increased expression of beta-defensins on SCC, adenovirus vectors with beta-defensin were transfected into SCC tissues in nude mice. The diameter of the tumor mass into which the gene was trandected was significantly smaller than that of the controls. The results indicate that beta-defensins themselves may posses a direct antitumor effect against SCC. In order to observe the antitumor effect of DNA vaccine with beta-defensins on lymphoma, the vaccine was injected into A20 lymphoma inBALB/c mice. The survival rate of the vaccination group was significantly longer than that of the control group. The results indicate that DNA vaccine with beta-defensins may be useful in the treatment of lymphoma.

Research Products

• [Journal Article] Increased expression of beta-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL/lpr mice (2007)
  • Author(s): Saitoh M, Kurashige Y, Yamaza ki M, Nishimura M, Nakamura S, Noro D, TakeshimaM, Araka wa T, Takuma T, Igarashi S, Ka ku T, Inoue T, Abiko Y
  • Journal Title: Med Mol Morph 40 Pages: 157-162
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Salivary defensins and their importance in oral health and disease(Review) (2007)
  • Author(s): Abiko Y, Saitoh M
  • Journal Title: Curr Pharm Design 13 Pages: 3065-3072
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Role of beta-defensin in oral epithelial health and disease(Award review) (2007)
  • Author(s): Abiko Y, Satoh M, Nishimura M, Yamazaki M, Sawamura D, Kaku T
  • Journal Title: Med Mol Morph 40 Pages: 179-184
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Increased expression of beta-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL/lpr mice. Med Mol Morph (2007)
  • Author(s): Saitoh, M., Kurashige, Y., Yamazaki, M., Nishimura, M., Nakamura, S., Noro, D., Takeshima, M., Arakawa, T., Takuma, T., Igarashi, S., Kaku, T., Inoue, T., Abiko, Y
  • Journal Title: Med Mol Morph 40 Pages: 157-162
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Salivary defensins and their importance in oral health and disease(Review) (2007)
  • Author(s): Abiko, Y., Saitoh, M
  • Journal Title: Curr Pharm Design 13 Pages: 3065-3072
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of beta-defensin in oral epithelial health and disease(Award review) (2007)
  • Author(s): Abiko, Y., Satoh, M., Nishimura, M., Yamazaki, M., Sawamura, D., Kaku, T
  • Journal Title: Med Mol Morph 40 Pages: 179-184
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Single-nucleotide Polymorphism(SNP) in B-Defensin 2 in a Japanese Population and an Effect of - 1029 SNP on Promoter Activity (2005)
  • Author(s): Kusano k, Abiko Y, Nishimura M, Arakawa T, Takeshima M, Fujimoto A, Takuma T, Kaku T
  • Journal Title: Oral Science International 2 Pages: 80-84
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Beta defensin-3 engineered epidermis shows highly protective effect for bacterial infection (2005)
  • Author(s): Sawamura D, Goto M, Shibaki A, Akiyama M, McMillan JR, A biko Y, Shimizu H
  • Journal Title: Gene Ther 12 Pages: 857-861
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Single-nucleotide Polymorphism(SNP) in 6-Defensin 2 in a Japanese Population and an Effect of - 1029 SNP on Promoter Activity (2005)
  • Author(s): Kusano, k., Abiko, Y., Nishimura, M., Arakawa, T., Takeshima, M., Fujimoto, A., Takuma, T., Kaku, T
  • Journal Title: Oral Science International 2 Pages: 80-84
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Beta defensin-3 engineered epidermis shows highly protective effect for bacterial infection (2005)
  • Author(s): Sawamura, D., Goto, M., Shibaki, A., Akiyama, M., McMillan, JR., Abiko, Y., Shimizu, H
  • Journal Title: Gene Ther 12 Pages: 857-61
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] C-terminal domain of human CAP18 antimicrobial peptide induces apotosis in oral squamous cell (2004)
  • Author(s): Okumura K, Itoh A, Isogai E, Hirose K, Hosokawa Y, Abiko Y, Shibata T, Hirata M, Isogai H
  • Journal Title: Cancer Lett 212 Pages: 185-194
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Correlated expression of human beta defensin-1,-2 and -3 mRNAs in gingival tissues of young children (2004)
  • Author(s): Saitoh M, Abiko Y, Shimabukuro S, Kusano K, Nishimura M, Arakawa T, Nakashima K, Takuma T, Kaku T, Igarashi S
  • Journal Title: Arch Oral Biol 49 Pages: 799-803
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effect of defensin peptides on eukaryotic cells primary epithelial cells,flibroblasts and squamous cell carcinoma cell lines (2004)
  • Author(s): Nishimura M, Abiko Y, Kurashi ge Y, Takeshima M, Yamazaki M, Kusano K, Saitoh M, Nakas hima K, Inoue T, Kaku T
  • Journal Title: J Dermatol Sci 36 Pages: 87-95
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 小児歯肉におけるヒトβディフェンシン1-2-3mRNAの発現及び局在 (2004)
  • Author(s): 斉藤 正人、島袋 鎮太郎、坂口 也子、桜井 有子、野呂 大輔、菅谷 裕行、五十嵐 清治、西村 学子、安彦 善裕
  • Journal Title: 小児歯科学雑誌 42 Pages: 69-74
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 口腔扁平苔癬におけるβディフェンシン2の発現と臨床病理学的所見との関連についての検討 (2004)
  • Author(s): 野口 忠秀, 神部 芳則, 草間 幹夫, 安彦 善裕, 賀来 亨
  • Journal Title: 日本口腔粘膜学会雑誌 10 Pages: 6-10
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] C-terminal domain of human CAP18 antimicrobial peptide induces apotosis in oral squamous cell (2004)
  • Author(s): Okumura, K., Itoh, A., Isogai, E., Hirose, K., Hosokawa, Y., Abiko, Y., Shibata, T., Hirata, M., Isogai, H
  • Journal Title: Cancer Lett 212 Pages: 185-94
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Correlated expression of human beta defensin-1, -2 and -3 mRNAs in gingival tissues of young children (2004)
  • Author(s): Saitoh, M., Abiko, Y., Shimabukuro, S., Kusano, K., Nishimura, M., Arakawa, T., Nakashima, K., Takuma, T., Kaku, T., Igarashi, S
  • Journal Title: Arch Oral Biol 49 Pages: 799-803
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of defensin peptides on eukaryotic cells : primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines (2004)
  • Author(s): Nishimura, M., Abiko, Y., Kurashige, Y., Takeshima, M., Yamazaki, M., Kusano, K., Saitoh, M., Nakashima, K., Inoue, T., Kaku, T
  • Journal Title: J Dermatol Sci 36 Pages: 87-95
  • Description: 「研究成果報告書概要(欧文)」より