Induction of reparative dentin by Enamel Proteins

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 16591921
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Meikai University (2007); Showa University (2004-2006)
• Principal Investigator: NAKAMURA Yukio Meikai University, School of Dentistry, Professor (40207931)
• Co-Investigator(Kenkyū-buntansha): WATANABE Haruji Showa University, School of Dentistry, Assistant Professor (90191783) ; MASUDA Yoshiko Showa University, School of Dentistry, Assistant Professor (10297038)
• Project Period (FY): 2004 – 2007
• Keywords: ameloblastin / pulp / dentin / wound healing / immunohistochemistry

Research Abstract

We have shown that enamel matrix derivative (EMD) enhanced reparative dentin formation was associated with a sequential expression of dentin-specific factors in the wounded pulp. Notably, in these studies, expression of pig ameloblastin was detected in pulpal cells in the wound area shortly following EMD application. To confirm a functional role for Ambn in dentinogenesis and to explore the potential use of Ambn for pulpal healing, we investigated if application of recombinant fusion Ambn (rAmbn) onto exposed pulp tissue could enhance pulpal healing and secondary dentin formation by histomorphometry and immunohistochemistry. In rAmbn-treated teeth, a substantial amount of newly formed reparative dentin was observed at the application site, completely bridging the pulpal wound. Dentin formation was also observed in Ca(OH)2 trated teeth; however, the amount of reparative dentin was significantly smaller than after rAmbn treatment. Immunohistochemistry confirmed that the new hard tissue formed was similar to dentin. The results suggest potential for rAmbn as a biologically active pulp-dressing agent for enhanced pulpal wound healing and reparative dentin formation after pulpotomy procedures.

Research Products

• [Journal Article] Analysis of surface roughness and microleakage of fissure sealants following organic debris removal with Carisolv (2008)
  • Author(s): Yamada Y, Hossain M, Shimizu Y, Kimura Y, MasudaY, Nakamura Y, Matsumoto K
  • Journal Title: Journal of dentistry 36(2) Pages: 130-137
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Analysis ofsurface roughness and microleakage of fissure sealants following organic debris removal with Carisolv (2008)
  • Author(s): Yamada, Y., Hossain, M., Shimizu, Y., Kimura, Y., Masuda, Y., Nakamura, Y., Matsumoto, K
  • Journal Title: Journal of dentistry 36(2) Pages: 130-137
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Artificial plaque removal with Carisolv system: a clinical approach (2007)
  • Author(s): Yamada Y, Hossain, KimuraY, Nakamura Y, Masuda Y, Shimizu Y, Matsumoto K
  • Journal Title: The Journal of clinical pediatric dentistry 31(3) Pages: 199-201
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Artificial plaque removal with Carisolv system : a clinical approach (2007)
  • Author(s): Yamada, Y., Hossain, KimuraY, Nakamura, Y., Masuda, Y., Shimizu, Y., Matsumoto, K
  • Journal Title: The Journal of clinical pediatric 31(3) Pages: 199-201
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Ameloblastin Fusion Proteinsによる修復象牙質の形成誘導について (2007)
  • Author(s): 中村 幸生, 松本 光吉
  • Organizer: 日本外傷歯学会
  • Place of Presentation: 愛知学院大学(名古屋)
  • Year and Date: 2007-10-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Induction of reparative dentin by Ameloblastin Fusion Proteins (2007)
  • Author(s): Nakamura, Y., Matsumoto, K
  • Organizer: Japan Association of Dental Traumatology
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2007-06-10
  • Description: 「研究成果報告書概要(欧文)」より