2006 Fiscal Year Final Research Report Summary
Characterization of Mallase's Epithelial Rest cell line and development to anti-tooth absorbing therapy.
| Project/Area Number |
16591926
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
TSUNODA Akira Kanagawa Dental College, Dentistry, Lecturer, 歯学部, 講師 (70236933)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Masahiro Osaka University, Graduate School dentistry, Graduate course, 大学院歯学研究科, 講師 (40215562)
TERANAKA Toshio Kanagawa Dental College, Dentistry, Professor, 歯学部, 教授 (60104460)
SUDA Naoto Tokyo Medical and Dental College, Dentistry, Lecturer, 大学院医歯学総合研究科, 講師 (90302885)
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| Project Period (FY) |
2004 – 2006
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| Keywords | ameloblast / epithelial rest / enamel / immortalization / root / development / dentin / tooth germ |
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| Research Abstract |
Mallase's Epithelial Rest cell (MMER) is an ameloblast that lined on a tooth root dentin. MMER found to express enamel protein such as amelogenin, however biological role of this cell is still unclear. Recently amelogenin knockout mice showed progressive tooth root absorption suggesting that enamel protein synthesized by MMER act to inhibit osteoclast activation on the tooth root surface. To investigate a biological role of MMER, we try to establish immortalized MMER cell line. MMER isolated from mice incisor root analog side, and immortalized with human papilloma virus E6 deleted with PDZ domain binding motif (MMER^<E6). MMER^<E6> able to grow more that population doubling 30, while MMER without immortalization stop to divide until population doubling 10. MMER expressed amelogenesis related gene such as enamelin, however no expression of amelogenenin was observed. These data indicated that MMER was successfully isolated, however culture condition that induced ameloblast differentiation should be required for investigating biological role of MMER.
On the other hand, we established immortalized mice dental epithelium cells (MDE) isolated form developing tooth germ at postnatal lday. MDE able to differentiate into amelobalst that expressed amelogenesis related genes such as amelogenin, ameloblastin and enamelin. These data indicating that MDE could serve as an experimental model for investigating function of MIMER.
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[Journal Article] Establishment of Immortalized Dental Follicle Cells for Generating Periodontal Ligament In Vivo.2007
Author(s)
T.Yokoi, M.Saito, T.Kiyono, S.Iseki, K.Kosaka, E.Nishida, T.Tsubakimoto, H.Harada, K.Eto, T.Noguchi, T.Teranaka
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Journal Title
Cell and Tissue Res. 327(2)
Pages: 301-311
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular mechanisms of Periodontal development, and its prospect of regenerative medicine for periodontium.2005
Author(s)
M.Saito, A.Tsunoda, T.Yokoi, S.Hattori, T.Tsubakimoto, E.Nishida, K.Kousaka, M.Toyoda, S.Sato, T.Noguchi, T.Teranaka.
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Journal Title
Bulletin of Kanagawa dental college. Vol.33(1)
Pages: 31-35
Description
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[Journal Article] Immortalization of cementoblast progenitor cells with Bmi-1 and TERT.2005
Author(s)
M.Saito, K.Handa, T.Kiyono, S.Hattori, T.Yokoi, T.Tsubakimoto, H.Harada, T.Noguchi, M.Toyoda, S.Sato, T.Teranaka
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Journal Title
J Bone Miner Res 20(1)
Pages: 50-57
Description
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[Journal Article] Alveolar Bone Marrow as a Cell Source for Regenerative Medicine : Differences between Alveolar and Iliac Bone Marrow Stromal Cells. J Bone Miner Res, 20(3), 399-409, 20052005
Author(s)
T.Matsubara K.Suardita, M.Ishii, M.Sugiyama, A.Igarashi, R.Oda, M.Nishimura,.Saito M, K.Nakagawa, K.Yamanaka, K.Miyazaki, M.Shimizu, K.Tsuji, K.Nakamura, Y.Kato.
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Journal Title
J Bone Miner Res 20(3)
Pages: 50-57
Description
「研究成果報告書概要(欧文)」より
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