2006 Fiscal Year Final Research Report Summary
Callus formation of membrous bone reconstruction using micro vascular endochondral bone
| Project/Area Number |
16591995
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kobe University |
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Principal Investigator |
YOKOO Satoshi Kobe University, Hospital affiliated to a medical school, Lecturer, 医学部附属病院, 講師 (00322206)
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| Co-Investigator(Kenkyū-buntansha) |
KOMORI Takahide Kobe University, Graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (50251294)
TERASHI Hiroto Kobe University, Hospital affiliated to a medical school, Associate Professor, 医学部附属病院, 助教授 (80217421)
UMEDA Masahiro Kobe University, Graduate School of Medicine, Associate Professor, 大学院医学系研究科, 助教授 (60301280)
ICHINOSE Akihiro Kobe University, Hospital affiliated to a medical school, Assistant Professor, 医学部附属病院, 助手 (90362780)
OJIMA Yasutaka Kobe University, Hospital affiliated to a medical school, Medical staff, 医学部附属病院, 医員 (40403240)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Dentistry / Tissue Engineering / Surgery |
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| Research Abstract |
Recently, plate-rich plasma (RPR) has been used clinically to promote bone regeneration and wound healing. However, no study has clearly reported on the activity of PRP after preparation. We established a protocol for the safe production of RPR from small quantities of blood.
Various conditions were used fir anticoagulants and centrifugal separation speed for blood sample obtained from 23 healthy subjects to investigate optimal concentration condition. Our results showed that when 20% ACD-A liquid was used as the anticoagulant, PRP was most efficiently obtained by double centrifugation at 800 r.p.m and 1300 r.p.m. The effective of PRP was confirmed by platelet aggregation reaction and the TGF-β content of PRP after centrifugation.
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