Teeth eruption and movement in tissue-engineered bone

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 16592039
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: SUSAMI Takafumi The University of Tokyo, Faculty of Medicine, Assistant Professor, 医学部附属病院, 助教授 (80179184)
• Co-Investigator(Kenkyū-buntansha): OGIHARA Yuji The University of Tokyo, Faculty of Medicine, Assistant, 医学部附属病院, 助手 (20345226) ; TAKATO Tsuyoshi The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (90171454) ; HOSHI Kazuto The University of Tokyo, Faculty of Medicine, visiting Assistant Professor, 医学部附属病院, 寄付講座教員 (30344451) ; OGASAWARA Toru The University of Tokyo, Faculty of Medicine, Contribute lectures staff, 医学部附属病院, 寄付講座教員 (20359623)
• Project Period (FY): 2004 – 2005
• Keywords: Dentistry / Regenerative Medicine / Bone Metabolism

Research Abstract

The purposes of this study were ; (1) how to make tissue-engineered bone in order to apply regenerative medicine to bone graft for CLP patients and (2) to establish the system(s) to evaluate such application from an orthodontic viewpoint.
First, to prepare a mesenchymal cell proliferation medium that does not contain fetal bovine serum (FBS), and that provides more than a 1000-fold increase in cell numbers within approximately a month, we investigated the combinational effects of 12 factors on the proliferation of human cells by analysis of variance in fractional factorial design. As a result, FGF-2, dexamethasone, insulin, and IGF-I possessed promotional effects on proliferation on chondrocytes, while the combination of FGF-2 with insulin or IGF-I synergistically enhanced the proliferation. Thus we concluded that such a combination of FGF-2 with insulin or IGF-I may be useful for promotion of auricular chondrocyte proliferation in a clinical application for cartilage regeneration. Next we discovered that the combination of BMP-2, insulin, and thyroid hormone (BIT) is ideal to deal with the problem of de-differentiation of the cell caused along with the cell proliferation culture. Then, we clarified the characteristics of some hydro-gels expected to be used in clinical application by studying their effects on cell proliferation or differentiation. Moreover, we reported that CGK2 plays an important role on chondrocyte differentiation and that the combination of Cdk4 and cyclin D1 could be used in bone regeneration.

Research Products

• [Journal Article] Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials. (2006)
  • Author(s): Yamaoka H. et al.
  • Journal Title: J Biomed Mater Res (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Assessment of dental arch relationships in Japanese patients with unilateral cleft lip and palate. (2006)
  • Author(s): Susami T. et al.
  • Journal Title: Cleft Palate-Craniofacial J 43 Pages: 96-102
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Suppression of Adjuvant-Induced Arthritic Bone Destruction by Cyclooxygenase-2 Selective Agents with and without InhibitoryPotency against Carbonic Anhydrase II. (2006)
  • Author(s): Katagiri M., Ogasawara T., Hoshi K., Chikazu D., Kimoto A., Noguchi M., Sasamata M., Harada S., Akama H., Tazaki H., Chung U., Takato T., Nakamura K., Kawaguchi H.
  • Journal Title: J Bone Miner Res 21 Pages: 219-227
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials (2006)
  • Author(s): Yamaoka, H., Asato, H., Ogasawara, T., Nishizawa, S., Takahashi, T., Nakatsuka, T., Koshima, I., Nakamura, K., Kawaguchi, H., Chung, U-I, Takato, T., Hoshi K.
  • Journal Title: J Biomed Mater Res (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Assessment of dental arch relationships in Japanese patients with unilateral cleft lip and palate. (2006)
  • Author(s): Susami, T., Ogihara Y., Matsuzaki M., Sakiyama M., Takato, T., Shaw WC, Semb G.
  • Journal Title: Cleft Palate-Craniofacial J 43(1) Pages: 96-102
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inhibition of Cdk6 expression through p38 MAP kinase is involved in differentiation of mouse prechondrocyte ATDC5. (2005)
  • Author(s): Moro T.et al.
  • Journal Title: J Cell Physiol 204 Pages: 927-933
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Mutation in cGMP-dependent protein kinase II causes dwarfism in a rat mutant KMI through uncoupling of proliferation and differentiation of chondrocytes. (2005)
  • Author(s): Chikuda H. et al.
  • Journal Title: J Bone Miner Metab 23 Pages: 200-204
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The canonical Wnt signaling pathway promotes chondrocyte differentiation in a Sox9-dependent manner. (2005)
  • Author(s): Yano F.et al.
  • Journal Title: Biochem Biophys Res Commun 333 Pages: 1300-1308
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Combination of platelet-rich plasma and cyclooxygenase-2 inhibitor potently stimulates bone marrow stromal cell proliferation in vivo. (2005)
  • Author(s): Chikazu D. et al.
  • Journal Title: Asian J Oral Maxillofac Surg 17 Pages: 81-87
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Synergistic effects of FGF-2 with insulin or IGF-I on the proliferation of human auricular chondrocytes. (2005)
  • Author(s): Takahashi T. et al.
  • Journal Title: Cell Transplant 14 Pages: 683-693
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Suppression of Adjuvant-Induced Arthritic Bone Destruction by Cyclooxygenase-2 Selective Agents with and without Inhibitory Potency against Carbonic Anhydrase II. (2005)
  • Author(s): Katagiri M. et al.
  • Journal Title: J Bone Miner Res 21 Pages: 219-227
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Inhibition of Cdk6 expression through p38 MAP kinase is involved in differentiation of mouse prechondrocyte ATDC5. (2005)
  • Author(s): Moro, T., Ogasawara, T., Chikuda, H., Ikeda, T., Ogata, N., Maruyama, Z., Komori, T., Hoshi, K., Chung, U.I., Nakamura, K, Okayama, H., Kawaguchi, H.
  • Journal Title: J Cell Physiol 204 Pages: 927-933
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mutation in cGMP-dependent protein kinase IIcauses dwarfism in a rat mutant KMI through uncoupling of proliferation and differentiation of chondrocytes. (2005)
  • Author(s): Chikuda, H., Kugimiya, F., Hoshi, K., Ikeda, T., Ogasawara, T., Kamekura, S., Ogata, N., Nakamura, K., Chung, U.I., Kawaguchi, H.
  • Journal Title: J Bone Miner Metab 23 Pages: 200-204
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The canonical Wnt signaling pathway promotes chondrocyte differentiation in a Sox9-dependent manner. (2005)
  • Author(s): Yano, F., Kugimiya, F., Ohba, S., Ikeda, T., Chikuda, H., Ogasawara, T., Ogata, N., Takato, T., Nakamura, K., Kawaguchi, H., Chung, U.I.
  • Journal Title: Biochem Biophys Res Commun 333 Pages: 1300-1308
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Combination of platelet-rich plasma and cyclooxygenase-2 inhibitor potently stimulates bone marrow stromal cell proliferation in vivo. (2005)
  • Author(s): Chikazu, D., Ohba, S., Ogasawara, T., Saijo, H., Mori, Y., Tomizuka, K., Kawaguchi, H, Yonehara, Y., Susami, T., Takato, T.
  • Journal Title: Asian J Oral Maxillofac Snug 17(2) Pages: 81-87
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Synergistic effects of FGF-2 with insulin or IGF-I on the proliferation of human auricular chondrocytes. (2005)
  • Author(s): Takahashi, T., Ogasawara, T., Kishimoto, J., Liu, G., Asato, H., Nakatsuka, T., Uchinuma, E., Nakamura, K., Kawaguchi, H., Takato, T., Hoshi, K.
  • Journal Title: Cell Transplant 14 Pages: 683-693
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Osteoclast differentiation by RANKL requires NF-κB-mediated downregulation of cyclin-dependent kinase 6(Cdk6). (2004)
  • Author(s): Ogasawara T. et al.
  • Journal Title: J Bone Miner Res 19 Pages: 1128-1136
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Bone morphogenetic protein 2-induced osteoblast differentiation requires Smad-mediated down-regulation of Cdk6. (2004)
  • Author(s): Ogasawara T. et al.
  • Journal Title: Mol Cell Biol 24 Pages: 6560-6568
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cyclic GMP-dependent protein kinase II is a molecular switch from proliferation to hypertrophic differentiation of chondrocytes. (2004)
  • Author(s): Chikuda H. et al.
  • Journal Title: Genes Dev 18 Pages: 2418-2429
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Osteoclast differentiation by RANKL requires NF-kappaB-mediated downregulation of cyclin-dependent kinase 6 (Cdk6). (2004)
  • Author(s): Ogasawara, T., Katagiri, M., Yamamoto, A., Hoshi, K., Takato, T., Nakamura, K., Tanaka, S., Okayama, H., Kawaguchi, H.
  • Journal Title: J Bone Miner Roe 19 Pages: 1128-1136
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bone morphogenetic protein 2-induced osteoblast differentiation requires Smad-mediated down-regulation of Cdk6. (2004)
  • Author(s): Ogasawara, T., Kawaguchi, H., Jinno, S., Hoshi, K., Itaka, K., Takato, T., Nakamura, K., Okayama, H.
  • Journal Title: Mol Cell Biol 24 Pages: 6560-6568
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cyclic GMP-dependent protein kinase II is a molecular switch from proliferation to hypertrophic differentiation of chondrocytes. (2004)
  • Author(s): Chikuda, H., Kugimiya, F., Hoshi, K., Ikeda, T., Ogasawara, T., Shimoaka, T., Kawano, H., Kamekura, S., Tsuchida, A., Yokoi, N., Nakamura, K., Komeda, K, Chung, U.I., Kawaguchi, H.
  • Journal Title: Genes Dev 18 Pages: 2418-2429
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 口唇口蓋裂のチーム医療. (2005)
  • Author(s): 須佐美隆史, 米原啓之 編
  • Total Pages: 206
  • Publisher: 金原出版
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 顎口腔外傷のチーム医療. (2005)
  • Author(s): 米原啓之, 須佐美隆史 編
  • Total Pages: 143
  • Publisher: 金原出版
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Team approach on cleft Lip Palate (2005)
  • Author(s): Susami, T., Yonehara Y.
  • Total Pages: 206
  • Publisher: Kanehara-shuppan
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Team approach on traumatic injuries to the oral-maxillofacial region (2005)
  • Author(s): Yonehara Y., Susami, T.
  • Total Pages: 143
  • Publisher: Kanehara-shuppan
  • Description: 「研究成果報告書概要(欧文)」より