• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2005 Fiscal Year Final Research Report Summary

Prion-conformation-specific human antibodies established from phage display library.

Research Project

Project/Area Number 16613008
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 食の安全
Research InstitutionKagoshima University

Principal Investigator

HASHIGUCHI Shuhei  Kagoshima University, Faculty of Engineering, Research Associate, 工学部, 助手 (40295275)

Co-Investigator(Kenkyū-buntansha) SUGIMURA Kazuhisa  Kagoshima University, Faculty of Engineering, Professor, 工学部, 教授 (80127240)
ITO Yuji  Kagoshima University, Faculty of Engineering, Assistant Professor, 工学部, 助教授 (60223195)
Project Period (FY) 2004 – 2005
KeywordsPhage display / Human antibody / Prion / Antibody engineering / Conformation / Food / Diagnosis / Neurodegenerative disorders
Research Abstract

Recombinant prion protein (PrP : aa23-231) was refolded into soluble from rich in β-structure at pH 4.0, as determined by circular dichroism (CD). The soluble β form (β-PrP) exhibited partial resistance to proteinase K digestion, and composed of small spherical particles (3 nm in diameter estimated by atomic force microscopy). Direct panning was done against β-PrP using a human scFv (single chain Fv)-displaying phage library. After two rounds of panning, we isolated two clones (β-PrP7 and β-PrP30) specifict to β-PrP. We tested the binding activity of these clones against β-PrP preparations that had been incubated at 4℃ for 0, 4 or 14 days. The binding activity of both clones became stronger depending on the incubation time. These results indicate that the content of β-PrP conformers recognized by β-PrP7 or β-PrP30 has increased dynamically in the solution at 4℃, which process is detectable by these particular conformation-specific scFv clones.

  • Research Products

    (2 results)

All 2005

All Journal Article (1 results) Book (1 results)

  • [Journal Article] Prion-conformation-specific human antibodies established from phage display library.2005

    • Author(s)
      Shuhei Hashiguchi, Mayumi Yamamoto, Sho Kitamoto, Toshihiro Nakashima, Hitoki Yamanaka, Daisuke Ishibashi, Suehiro Sakaguchi, Shigeru Katamine, Yyji Ito, Kazuhisa Sugimura
    • Journal Title

      Prions : Food and Drug Safety (Ed.T.Kitamoto)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Prions : Food and Drug Safety(Ed T.Kitamoto)2005

    • Author(s)
      Shuhei Hashiguchi et al.
    • Total Pages
      2
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2007-12-13  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi