2005 Fiscal Year Final Research Report Summary
Analysis of Serum Proteins of Scrapie and BSE and Application to the Diagnosis.
| Project/Area Number |
16613010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食の安全
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| Research Institution | Rakuno Gakuen University |
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Principal Investigator |
YOKOTA Hiroshi Rakuno Gakuen University, School of Veterinary Medicine, Professor, 獣医学部, 教授 (90137414)
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| Co-Investigator(Kenkyū-buntansha) |
DOH-URA Katsumi Tohoku University, School of Medicine, professor, 医学部, 教授 (00263012)
MORI Seiichi Hokkaido Animal Research Center, Cheaf of Center, 場長 (10390824)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Scrapie / BSE / Serum Proteins / 2d-electrophoresis / Diagnosis |
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| Research Abstract |
The objective of this study is to find signals of mouse scrapie and BSE by comprehensive analysis of serum components. Several proteins were found to detect only in the sera from scrapie mice by 2-dementional electrophoresis. The detected proteins were analyzed by Ms/Ms and one of them was identified to antibodies against Fas protein expressed on the cell surface membrane. In BSE sera which were obtained from the Weybridge Veterinary Laboratory, several protein bands recognized by serum antibodies were found by Western blotting analysis. The antigens were extracted from the protein stops prepared by 2-clementional electrophoresis, and were analyzed by Ms.
These data indicate that autoantibodies were produced in the sera of not only scrapie mice but also BSE cows. In next study, we can consider to use these serum components diagnostic signals.
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[Journal Article] Differences in the metabolism of di(2-ethylhexyl) phthalate(DEHP) in several organs of mice,rats,and marmosets.2005
Author(s)
Ito Y, Yokota H, Wang R, Yamanoshita O, Ichihara G, Wang h, Kurata Y, Takagi K, Nakajima T.
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Journal Title
Arch Toxicol. 79
Pages: 147-154
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Differences in the metabolism of di(2-ethylhexyl) phthalate (DEHP) in several organs of mice, rats, and marmosets.2005
Author(s)
Ito Y, Yokota H, Wang R, Yamanoshita O, Ichihara G, Wang H, Kurata Y, Takagi K, Nakajima T.
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Journal Title
Arch Toxicol 79
Pages: 147-154
Description
「研究成果報告書概要(欧文)」より
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[Book] 獣医生化学2005
Author(s)
斉藤昌之, 鈴木喜彦, 横田博
Total Pages
234
Publisher
朝倉書店
Description
「研究成果報告書概要(和文)」より
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