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2005 Fiscal Year Final Research Report Summary

Analysis of Serum Proteins of Scrapie and BSE and Application to the Diagnosis.

Research Project

Project/Area Number 16613010
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 食の安全
Research InstitutionRakuno Gakuen University

Principal Investigator

YOKOTA Hiroshi  Rakuno Gakuen University, School of Veterinary Medicine, Professor, 獣医学部, 教授 (90137414)

Co-Investigator(Kenkyū-buntansha) DOH-URA Katsumi  Tohoku University, School of Medicine, professor, 医学部, 教授 (00263012)
MORI Seiichi  Hokkaido Animal Research Center, Cheaf of Center, 場長 (10390824)
Project Period (FY) 2004 – 2005
KeywordsScrapie / BSE / Serum Proteins / 2d-electrophoresis / Diagnosis
Research Abstract

The objective of this study is to find signals of mouse scrapie and BSE by comprehensive analysis of serum components. Several proteins were found to detect only in the sera from scrapie mice by 2-dementional electrophoresis. The detected proteins were analyzed by Ms/Ms and one of them was identified to antibodies against Fas protein expressed on the cell surface membrane. In BSE sera which were obtained from the Weybridge Veterinary Laboratory, several protein bands recognized by serum antibodies were found by Western blotting analysis. The antigens were extracted from the protein stops prepared by 2-clementional electrophoresis, and were analyzed by Ms.
These data indicate that autoantibodies were produced in the sera of not only scrapie mice but also BSE cows. In next study, we can consider to use these serum components diagnostic signals.

  • Research Products

    (21 results)

All 2005 2004 Other

All Journal Article (19 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Differences in the metabolism of di(2-ethylhexyl) phthalate(DEHP) in several organs of mice,rats,and marmosets.2005

    • Author(s)
      Ito Y, Yokota H, Wang R, Yamanoshita O, Ichihara G, Wang h, Kurata Y, Takagi K, Nakajima T.
    • Journal Title

      Arch Toxicol. 79

      Pages: 147-154

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Monocarboxylate transporter 1(MCT1) in the liver of pre-ruminant and adult bovines.2005

    • Author(s)
      Kirat D, Inoue H, Iwano H, Yokota H, Taniyama H, Kato S.
    • Journal Title

      Vet J. Aug 23(Epub ahead of print)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Movement and fixation of intestinal microbiota after administration of human feces to germfree mice.2005

    • Author(s)
      Kibe R, Sakamoto M, Yokota H, Ishikawa H, Aiba Y, Koga Y, Benno Y.
    • Journal Title

      Appl Environ Microbiol. 71

      Pages: 3171-3178

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression and distribution of monocarboxylate transporter 1(MCT1) in the gastrointestinal tract of calves.2005

    • Author(s)
      Kirat D, Inoue H, Iwano H, Hirayama K, Yokota H, Taniyama H, Kato S.
    • Journal Title

      Res Vet Sci. 79

      Pages: 45-50

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Isoform-specific expression and in duction of UDP-glucuronosyltransferase in immunoactivated peritonealmacrophages of the rat.2005

    • Author(s)
      Tochigi Y, Yamashiki N, Ohgiya S, Ganaha S, Yokota H.
    • Journal Title

      Drug Metab Dispos. 33

      Pages: 1391-1398

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] UDP-glucuronosyltransferase Isoforms Catalyzing Glucuronidation of Hydroxy-Polychlorinated Biphenyls in Rat.2005

    • Author(s)
      Daidoji T, Gozu K, Iwano H, Inoue H, Yokota H.
    • Journal Title

      Drug Metab Dispos. 33

      Pages: 1466-1476

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cytochrome P450 2E polymorphism in feline liver.2005

    • Author(s)
      Tanaka N, Shinkyo R, SakakiT, Kasamastu M, Imaoka S, Funae Y, Yokota H
    • Journal Title

      Biochim Biophys Acta. 1726(2)

      Pages: 194-205

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Differences in the metabolism of di(2-ethylhexyl) phthalate (DEHP) in several organs of mice, rats, and marmosets.2005

    • Author(s)
      Ito Y, Yokota H, Wang R, Yamanoshita O, Ichihara G, Wang H, Kurata Y, Takagi K, Nakajima T.
    • Journal Title

      Arch Toxicol 79

      Pages: 147-154

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Monocarboxylate transporter 1 (MCT1) in the liver of pre-ruminant and adult bovines.2005

    • Author(s)
      Kirat D, Inoue H, Iwano H, Yokota H, Taniyama H, Kato S.
    • Journal Title

      Vet J. Aug23[Epub ahead of print]

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Movement and fixation of intestinal microbiota after administration of human feces to germfree mice.2005

    • Author(s)
      Kibe R, Sakamoto M, Yokota H, Ishikawa H, Aiba Y, Koga Y, Benno Y.
    • Journal Title

      Appl Environ Microbio 71

      Pages: 3171-3178

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression and distribution of monocarboxylate transporter 1 (MCT1) in the gastrointestinal tract of calves.2005

    • Author(s)
      Kirat D, Inoue H, Iwano H, Hirayama K, Yokota H, Taniyama H, Kato S.
    • Journal Title

      Res Vet Sci 79

      Pages: 45-50

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Isoform-specific expression and induction of UDP-glucuronosyltransferase in immunoactivated peritoneal macrophages of the rat.2005

    • Author(s)
      Tochigi Y, Yamashiki N, Ohgiya S, Ganaha S, Yokota H.
    • Journal Title

      Drug Metab Dispos 33

      Pages: 1391-1398

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] UDP-glucuronosyltransferase Isoforms Catalyzing Glucuronidation of Hydroxy-Polychlorinated Biphenyls in Rat.2005

    • Author(s)
      Daidoji T, Gozu K, Iwano H, Inoue H, Yokota H.
    • Journal Title

      Drug Metab Dispos 33

      Pages: 1466-1476

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cytochrome P450 2E polymorphism in feline liver.2005

    • Author(s)
      Tanaka N, Shinkyo R, Sakaki T, Kasamastu M, Imaoka S, Funae Y, Yokota H
    • Journal Title

      Biochim Biophys Acta 1726(2)

      Pages: 194-205

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Glucuronidation 1-naphthol and excretion into the vein inperfused rat kidney.2004

    • Author(s)
      Narukawa J, Inoue H, Kato S, Yokota H.
    • Journal Title

      Drug Metab.Dispos. 32

      Pages: 758-761

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Maturation of the murine cecal microbiota as revealed by terminal restriction fragment length polymorphism and 16S rRNA gene clone libraries.2004

    • Author(s)
      Kibe R, Sakamoto M, HayashiH, Yokota H, Benno Y.
    • Journal Title

      FEMS Microbiology Letters 235

      Pages: 139-146

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Glucuronidation of 1-naphthol andexcretion into the vein in perfused rat kidney.2004

    • Author(s)
      Narukawa J, Inoue H, Kato S, Yokota H.
    • Journal Title

      Drug Metab. Dispos. 32

      Pages: 758-761

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Maturation of the murine cecal microbiota as revealed by terminal restriction fragment length polymorphism and 16S rRNA gene clone libraries.2004

    • Author(s)
      Kibe R, Sakamoto M, Hayashi H, Yokota H, Benno Y
    • Journal Title

      FEMS Microbiology Letters 235

      Pages: 139-146

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Enzyme and Outline of Metabolism

    • Author(s)
      Yokota H
    • Journal Title

      In "Veterinary Biochemistry" Saitoh M, Suzuki Y, Yokota H (Eds) Asakura, Tokyou, Japan

      Pages: 56-66

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 獣医生化学2005

    • Author(s)
      斉藤昌之, 鈴木喜彦, 横田博
    • Total Pages
      234
    • Publisher
      朝倉書店
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 体外診断キット及び体外診断方法2004

    • Inventor(s)
      横田 博, 堂浦克美
    • Industrial Property Rights Holder
      横田 博, 堂浦克美, 伊藤ハム(株)
    • Patent Publication Number
      出願番号:特原2004-216510, 公開番号:特開2006-038536
    • Filing Date
      2004-07-23
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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