2005 Fiscal Year Final Research Report Summary
Clarification of molecular mechanism of Jet Lag
| Project/Area Number |
16614008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
睡眠学
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| Research Institution | Kobe University |
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Principal Investigator |
MASUBUCHI Satoru Kobe University, Graduate School of medicine, Research Associate, 大学院・医学系研究科, 助手 (80362771)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMURA Hitoshi Kobe University, Graduate School of medicine, Professor, 大学院・医学系研究科, 教授 (60158813)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Jet lag syndrome / Clock genes |
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| Research Abstract |
We found that Per1 knockout (Per1-/-) mouse is a jet lag model animal which can re-entrain to experimental new time zone earlier. During the lighting task PER2 protein, core clock protein, rhythm in the master clock (SCN) moves largely without changing the Per2 mRNA increase. This protein level regulation of PER2 by PER1 is a cause of Jet Lag. In addition, we found Per1-/- mouse can hardly entrain to experimental long photoperiod for nocturnal life (with skeleton photoperiod, low light intensity). This result suggests that Per1 gene aided nocturnal survival life of ancestral mammals.
We next searched the transcriptional profiles of genes in the SCN during the dissociation between Per2 protein and mRNA in Per1-/- mouse. To sample SCN precisely, we established inbred line of Per1-/- mouse (backcrossed for ten times). We found largely induced genes by jet lag task in Per1-/- mouse by PCR subtraction technique and confirmed these movements of gene expressions by in situ hybridization.
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