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2018 Fiscal Year Annual Research Report

CRISPR/Cas9封入高分子集合体の構築と生体内デリバリーへの挑戦

Research Project

Project/Area Number 16F16355
Research InstitutionThe University of Tokyo

Principal Investigator

宮田 完二郎  東京大学, 大学院工学系研究科(工学部), 准教授 (50436523)

Co-Investigator(Kenkyū-buntansha) MIN HYUN SU  東京大学, 工学(系)研究科(研究院), 外国人特別研究員
Project Period (FY) 2016-10-07 – 2019-03-31
KeywordsCas9 / messenger RNA delivery / polyplex
Outline of Annual Research Achievements

Cas9 activities of various cationic polymers were evaluated by luciferase-based Cas9 activity system. This assay consists of three different plasmids - sgRNA-expressing plasmid, Stop codon-contained luciferase-expressing plasmid, and luciferase donor DNA plasmid, which were kindly donated by Professor Moritoshi Sato (UTokyo). A cationic polymer (PAsp(OCT/DET)) was complexed with Cas9 messenger RNA and then the resulting polyplexes were transfected into human cervical cancer (HeLa) cells. The Cas9 activities were measured by a luminescence microplate reader. The cationic polymer elicited the similar luminescence intensity compared to commercially available transfection agent, lipofectamine 3000. This result indicates that the new cationic polymer is promising for in vitro Cas9 mRNA transfection.

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

  • Research Products

    (5 results)

All 2019 Other

All Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (1 results)

  • [Presentation] リガンド搭載高分子ミセルの構築と核酸医薬デリバリーへの展開2019

    • Author(s)
      宮田完二郎, ミン ヒョンス, イ ウ, 内藤瑞, キム ヒョンジン, 片岡一則
    • Organizer
      第67回高分子討論会
  • [Presentation] 抗体リガンド搭載高分子ミセルの構築とがんへの核酸医薬デリバリー2019

    • Author(s)
      宮田完二郎, ミン ヒョンス, キム ヒョンジン, 内藤瑞, 松村保広, 片岡一則
    • Organizer
      日本核酸医薬学会第4回年会
  • [Presentation] Smart oligonucleotide delivery by polymer nanotechnology2019

    • Author(s)
      K. Miyata
    • Organizer
      The 35th International Conference of Photopolymer Science and Technology
    • Int'l Joint Research / Invited
  • [Presentation] Development of thiol-crosslinked micelle for systemic delivery of antisense oligonucleotide to treat brain diseases2019

    • Author(s)
      H. -J. Kim, H. -S. Min, K. Katsushima, Y. Kondo, K. Miyata, K. Kataoka
    • Organizer
      第67回高分子年次大会
  • [Remarks] 宮田研ホームページ

    • URL

      http://www.bmm.t.u-tokyo.ac.jp/index.html

URL: 

Published: 2019-12-27  

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