2016 Fiscal Year Annual Research Report
目標に向かう空間ナビゲーションのための視床下部ー海馬回路の機能解明
| Project/Area Number |
16F16386
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
McHugh Thomas 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (50553731)
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| Co-Investigator(Kenkyū-buntansha) |
CHEN SHUO 国立研究開発法人理化学研究所, 脳科学総合研究センター, 外国人特別研究員
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| Project Period (FY) |
2016-10-07 – 2018-03-31
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| Keywords | hypothalamus / hippocampus / DG / spatial working memory |
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| Outline of Annual Research Achievements |
Optogenetic stimulation of SuM terminals in the DG does not impair social behavior, however we have found that inhibition of these projections leads to profound deficits in spatial working memory, while stimulation of the terminals impaired context habituation. In vivo recordings of hippocampal physiology during T-maze behavior combined with optogenetics suggests that silencing of this input impairs trajectory-specific firing rate modulation of CA1 pyramidal cells in a manner that correlates with an increase in error trials. These data establish the SuM inputs as a novel modulator of hippocampal spatial memory and we are currently conducting analyses to link the behavioral and single unit phenotypes to alterations in hippocampal oscillations and dynamic information routing in the circuit.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
The project has developed several interesting lines of inquiry. The main focus on the role of the SuM/Dg circuit in spatial working memory has lead to clear behavioral and physiological phenotypes. In related work we have begun to examine the SuM projections to the medial septum and have striking and differential data showing that optogenetic stimulation of these fibers induces high speed movement and triggers a strong increase in the theta oscillation. these unexpected data will lead to a parallel series of experiments.
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| Strategy for Future Research Activity |
We are currently focused on adding more In vivo recordings of hippocampal physiology during manipulation of the SuM fibres in both the DG and medial septum. The effort this fiscal year will be to complete data collection and begin detailed analyses.
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