2018 Fiscal Year Final Research Report
Development of cell-free biosystems for artificial cells
| Project/Area Number |
16H02089
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Nanobioscience
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Ueda Takuya 東京大学, 大学院新領域創成科学研究科, 教授 (80184927)
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Keywords | リポソーム / PURE system / ATP合成酵素 / バクテリオロドプシン / 光合成 / 細胞分裂 / リボソーム / tRNA |
|---|
| Outline of Final Research Achievements |
Aiming at artificial cells, biosystems based on the reconstituted gene expression system, PURE system, have been developed. The proteins involved in the cell division system were synthesized in liposomes and liposomes appeared to deform. By expressing ATP synthetase and bacteriorhodopsin in liposome, a light-dependent energy production system was constructed. For the small subunit of ribosomes, an assembly system under the physiological conditions in the presence of biosynthetic factors was constructed. For the large subunit, we succeeded in reconstituting subunits with protein synthesis activity from individually purified ribosomal proteins and rRNA. We also developed a PURE system based on transcribed tRNA molecules.
|
| Free Research Field |
生化学
|
| Academic Significance and Societal Importance of the Research Achievements |
人工細胞は、今後の生命科学の中心的な流れである合成生物学の目標である。本研究では、DNAから分裂やエネルギー生産をする生命システムを作製した。また、遺伝子発現系を増殖させるために、リボソームのアセンブリー系とDNAから機能をもった tRNA分子の合成に成功した。これらの研究は、分子から細胞を創ろうとする試みの基盤技術を提供するものである。
|
