2016 Fiscal Year Annual Research Report
Molecular mechanisms for IgA-mediated regulations of host-microbiota symbiosis
| Project/Area Number |
16H02632
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
ファガラサン シドニア 国立研究開発法人理化学研究所, 統合生命医科学研究センター, チームリーダー (00391970)
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| Co-Investigator(Kenkyū-buntansha) |
服部 正平 早稲田大学, 理工学術院, 教授(任期付) (70175537)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | IgA |
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| Outline of Annual Research Achievements |
Our aim for FY 2016 was to perform experiments that would contribute to understanding of the roles played by IgA in regulating the gut bacterial communities. We aimed at characterizing in depth the physiology of a healthy microbiota upon transplantation to normal or immune-deficient germ-free mice. We planned to evaluate the composition, metabolic and immune activities of bacterial communities along the gastrointestinal tract of germ-free mice colonized with microbiota from normal mice.
So far DNA sequencing results indicate that even when identical microbiota behave differently when transplanted into normal or immune deficient hosts. The pattern of IgA staining evaluated by FCAS was also found to be different in mice capable or not capable of generating a selected repertoire of IgA plasma cells. We also found that at the systemic levels, the metabolic profiles between colonized mice and germ-free mice are profound.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
The progress was a bit slowed down lately by the departure of the postdoctoral fellow who conducted most of the experiments. We will be hiring a new postdoc stating from June 2017 to continue the experiments proposed in this grant application.
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| Strategy for Future Research Activity |
We observed that upon transplantation only a fraction of the bacterial community grafted into the recipient mice, regardless whether the mice were immune competent or immune deficient. This observation forces us to reevaluate a bit the proposal. While the data obtained so far looks great, it only reflects a partial microbiome reconstitution. We are currently reanalyzing, reassessing the results and soon we will make a decision as to how we will proceed with the experiments. Meanwhile were will make effort to set up the conditions for measuring the metabolome and if possible phosphoproteome. We also aim at evaluating bacterial communities at the transcriptomic level. This is going to be very important, as we are convinced that the most important factors by which bacterial are regulating the immune system and the body physiology relate with what they metabolically produce or help producing by the host cells, including the immune and epithelial cells.
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