Development of intraoperative diagnostic imaging technology for detecting residual breast cancer cells

2019 Fiscal Year Final Research Report

• Project/Area Number: 16H02894
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Life / Health / Medical informatics
• Research Institution: Hirosaki University
• Principal Investigator: NOZAKA HIROYUKI 弘前大学, 保健学研究科, 講師 (80302040)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 病理診断学 / 乳がん / 迅速診断

Outline of Final Research Achievements

Chemiluminescence is one of highly sensitive technology for protein expression assay in immunobiochemistry. Chemiluminescent Enzyme Immunoassay (CLEIA) is more sensitive than fluorescent enzyme immunoassays, and it is used as a diagnostic technology for the detection of infectious diseases. On the contrast, pathological diagnosis also requires highly sensitive antigen detection technology, but chemiluminescence technology has not been applied to immunohistochemistry. The aim of this study is the development of a new highly sensitive screening system for cancer cells and the application of chemiluminescence method. CIHC method has excellent sensitivity and specificity, and it was useful for screening of cancer cells. This screening system has a simple structure and can be easily downsized. Our system contributes to the advancement of immunohistochemistry.

Free Research Field

医用情報工学

Academic Significance and Societal Importance of the Research Achievements

本研究によりCLEIA技術を用いたCIHC法は感度と特異性に優れており,乳がん細胞のスクリーニングに有用であることが示唆された。化学発光の減衰は蛍光色素に比較して非常に遅く,最大24時間維持され,測定には励起光源やバンドパスフィルターを必要としないことが大きなメリットである。そのため,システムの小型化も容易である。今後この化学発光技術は術中迅速診断分野の進歩に貢献できるものと考えられ,さらにAI技術を加えることで,より小型のスマートポイントオブケアテスト（POCT）デバイス化も期待できる。