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2018 Fiscal Year Final Research Report

Improvement of medaka embryo toxicity assay by a novel pulsed power electroporation system

Research Project

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Project/Area Number 16H02990
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental risk control and evaluation
Research InstitutionAriake National College of Technology

Principal Investigator

TOMINAGA Nobuaki  有明工業高等専門学校, 創造工学科, 教授 (30227631)

Co-Investigator(Kenkyū-buntansha) 高橋 圭介  東邦大学, 薬学部, 准教授 (60380854)
大河平 紀司  有明工業高等専門学校, 創造工学科, 准教授 (60629210)
有薗 幸司  熊本県立大学, 環境共生学部, 教授 (70128148)
石橋 弘志  愛媛大学, 農学研究科, 准教授 (90403857)
Project Period (FY) 2016-04-01 – 2019-03-31
Keywords胚毒性試験 / メダカ / 高電界ナノパルス / ネオニコチノイド
Outline of Final Research Achievements

In this study, we improved the fish embryo toxicity assay using the nano-second pulsed electric field and medaka embryo. We achieved successfully efficient chemical incorporation to medaka egg by our original developed nano-second pulsed electroporation system without any adverse effects. Then we evaluated neonicotinoid chemicals and its metabolites. According to the results, neonicotinoids, were not only parent compounds but also metabolites, showed some developmental toxicity against medaka embryo. Moreover, various genes were affected gene expression even at lower concentration, which were not shown any phenotypic alteration.

Free Research Field

環境生物工学

Academic Significance and Societal Importance of the Research Achievements

従来の動物実験は,多くの制限があり,代替法の開発が期待されている.本研究の成果から,メダカ受精卵に効率よく化学物質を導入し,高感度に影響評価をできたことは,新たな動物代替法の可能性を示せた.代替動物実験で多くの化学物質の評価が行えるようになることは社会的意義がある.また,環境中に存在する化学物質にとどまらず,医薬品の開発等にも応用が期待される.メダカは脊椎動物であり,ヒトや他の生物との相同性も高いことから,作用機序のメカニズムが分子レベルで明らかにでき,学術的にも意義がある.

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Published: 2020-03-30  

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