2018 Fiscal Year Final Research Report
Methanol economy pioneered by C1-microorganisms: development of basic technologies utilized in low-carbon and sustainable society
| Project/Area Number |
16H02997
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Design and evaluation of sustainable and environmental conscious system
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Research Collaborator |
SAKAI Yasuyoshi
OKU Masahide
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 遺伝子発現制御 / 転写因子 / シグナル伝達 / 異種タンパク質生産 / メタノール / メタノール資化性酵母 / Methylobacterium |
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| Outline of Final Research Achievements |
C1-microorganisms that can utilize C1 compounds, such as methane and methanol, can be used for the bioprocess in the low carbon society “methanol economy”. In this project, we investigated molecular basis of C1-metabolism in C1-microorganisms and its application. We elucidated novel molecular functions of transcriptional regulators and signal transducers responsible for methanol-induced gene expression in methylotrophic yeasts which are used for heterologous protein production. Furthermore, we developed the basic technology of methanol production from biomass-derived sugar compounds and elucidated cellular functions relating to plant colonization of methylotrophic bacteria.
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| Free Research Field |
応用微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
詳細な分子機構が不明であった強力なメタノール誘導性遺伝子発現の分子基盤およびメタノール応答性転写活性化機構に関して、メタノール感知に関わるセンサータンパク質を初めて同定するとともに、発現制御の新規な機構を明らかにすることができた。本研究で解明したC1微生物代謝生理機能の分子基盤や有用物質生産のための基盤技術は、持続可能システムとしての低炭素・循環型物質生産プロセスに活用することができる。
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