On the role of amino acids in anoxic methane oxidation at sub-seafloor environment

2016 Fiscal Year Annual Research Report

• Project/Area Number: 16H04083
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: 高野 淑識 国立研究開発法人海洋研究開発機構, 生物地球化学研究分野, 主任研究員 (80399815)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: 分子レベルの多次元同位体解析（炭素・窒素） / メタノトロフィーと放射性炭素年代の計測 / ANME-sepcific 加速器質量分析

Outline of Annual Research Achievements

(1) アミノ酸の代謝プロセスの追跡検証を引き続き進め、炭素だけでなく窒素代謝にフォーカスした分子レベルの多次元解析（delta13C&delta15N）を進めた。クロマトグラフィーおよび質量分析法を中心にした有機化学的な解析に加えて、次世代シーケンサーを用いた代謝マップの構築を進め、昨年までの研究成果の一部を国際会議で発表した。
-Association for the Sciences of Limnology and Oceanography (ALSO) in March-2017.
-Goldschmidt 2016 in June-2016.

(2) メタノトロフィーからの放射性炭素年代の計測：メタンの起源・放射性炭素年代・プロセスを正確に記載するため、新たに、加速器質量分析装置を用いた放射性炭素年代測定法（ANME Cell -Specific Radiocarbon Analysis）を取り入れた（共同研究先：東京大学 大気海洋研究所 高解像度環境解析研究センター）。黒海における光合成系との比較（cf. Kusch et al., 2010. Biogeosciences 7, 4105-4118.; Wakeham, and McNichol, 2014. Biogeosciences 11, 6895-6914.）を行ったところ、ANME-specificな放射性炭素年代から明確なメタンの起源推定に世界で初めて成功した。また、分取クロマトグラフィーの最先端のハイライト研究について、依頼講演を数件受けた。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

本研究成果について、国際会議などでの招待講演、論文掲載・投稿を継続して行っていることから、おおむね研究計画は進展しているといえる。

Strategy for Future Research Activity

技術スタッフの部分的雇用による技術補填と多次元同位体標準試料による分析手法開発を進める。

Research Products

• [Int'l Joint Research] ドイツ連邦地質調査所（BGR）/ハンブルグ大学(ドイツ)
  • Country Name: GERMANY
  • Counterpart Institution: ドイツ連邦地質調査所（BGR）/ハンブルグ大学
• [Int'l Joint Research] メルボルン大学(オーストラリア)
  • Country Name: AUSTRALIA
  • Counterpart Institution: メルボルン大学
• [Journal Article] Fractionation of nitrogen isotopes during amino acid metabolism in heterotrophic and chemoautotrophic microbes across Eukarya, Bacteria, and Archaea: Effects of nitrogen sources and metabolic pathways. (2017)
  • Author(s): Yamaguchi, T.Y., Chikaraishi, Y., Takano, Y., Ogawa, N.O., Imachi, H., Yokoyama, Y. and Ohkouchi, N.
  • Journal Title: Organic Geochemistry Volume: in press Pages: in press
  • DOI: 10.1016/j.orggeochem.2017.04.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nitrogen isotopic fractionation in ammonia during adsorption on silicate surface. (2017)
  • Author(s): Sugahara, H., Takano, Y., Ogawa, N.O., Chikaraishi, Y. and Ohkouchi, N.
  • Journal Title: ACS Earth and Space Chemistry Volume: 1 Pages: 24-29
  • DOI: 10.1021/acsearthspacechem.6b00006.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Intra-trophic isotopic discrimination of 15N/14N for amino acids in autotrophs: implications for nitrogen dynamics in ecological studies. (2017)
  • Author(s): Takizawa, Y., Dharampal, S.P., Steffan, S.A., Takano, Y., Ohkouchi, N. and Chikaraishi, Y.
  • Journal Title: Ecology and Evolution Volume: 7 Pages: 2916-2924
  • DOI: 10.1002/ece3.2866.
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Deuterium fractionation on the formation of amino acids by photolysis of interstellar ice analogues containing deuterated methanol. (2016)
  • Author(s): Oba, Y., Takano, Y., Watanabe, N. and Kouchi, A.
  • Journal Title: The Astrophysical Journal Letters Volume: 827 Pages: L18 (pp.7)
  • DOI: 10.3847/2041-8205/827/1/L18.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Distributions and compound-specific isotopic signatures of sedimentary chlorins reflect the composition of photoautotrophic communities and their carbon and nitrogen sources in Swiss lakes and the Black Sea. (2016)
  • Author(s): Naeher, S., Suga, H., Ogawa, N.O., Takano, Y., Schubert, C.J., Grice, K. and Ohkouchi, N.
  • Journal Title: Chemical Geology Volume: 443 Pages: 198-209
  • DOI: 10.1016/j.chemgeo.2016.04.029.
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 「アーリーアース・ケミストリー（Early Earth Chemistry）」のプロローグ (2016)
  • Author(s): 古川 善博, 高野 淑識
  • Journal Title: 地球化学(Geochemistry) Volume: 50 Pages: 117-119
  • DOI: 10.14934/chikyukagaku.50.117
  • Peer Reviewed / Open Access
• [Presentation] Methane cycle in sub-seafloor environment: insight from key organic molecules in archaeal methanogenesis and methanotrophy 【INVITED】 (2017)
  • Author(s): Takano Y., Kaneko M., Chikaraishi Y., Ogawa O. N., Ohkouchi N.
  • Organizer: Association for the Sciences of Limnology and Oceanography (ALSO)
  • Place of Presentation: Honolulu Hawaii Convention Center, USA
  • Year and Date: 2017-02-28 – 2017-03-01
  • Int'l Joint Research / Invited
• [Presentation] COENZYME F430: A NOVEL BIOMARKER FOR METHANE CYCLE. (2017)
  • Author(s): Kaneko, M. Takano, Y. Sakata, S. Ohkouchi, N.
  • Organizer: Association for the Sciences of Limnology and Oceanography (ALSO)
  • Place of Presentation: Honolulu Hawaii Convention Center, USA
  • Year and Date: 2017-02-28 – 2017-03-01
  • Int'l Joint Research
• [Presentation] d26MG OF THE CHLOROPIGMENTS FROM OXYGENIC AND ANOXYGENIC PHOTOTROPHIC BACTERIA INSIDE THE BENTHIC MICROBIAL MAT OF THE TRAPANI SOLAR SALTERNS (ITALY). (2017)
  • Author(s): Isaji, Y. Yoshimura, T. Araoka, D. Kuroda, J. Ogawa, N. O. Takano, Y. et al.
  • Organizer: Association for the Sciences of Limnology and Oceanography (ALSO)
  • Place of Presentation: Honolulu Hawaii Convention Center, USA
  • Year and Date: 2017-02-28 – 2017-03-01
  • Int'l Joint Research
• [Presentation] Takano Y., Chikaraishi Y., Kruger M., and Ohkouchi N. (2016)
  • Author(s): Revealing the blackbox since 1999: 13C-depleted amino acids and lipids in deep-sea methane biogeochemistry.
  • Organizer: Goldschmidt 2016
  • Place of Presentation: Pacifico Yokohama（神奈川県横浜市）
  • Year and Date: 2016-06-26 – 2016-06-29
  • Int'l Joint Research
• [Presentation] Catabolic production in plants: significant influence for CHN isotopes in lipids and amino acids. (2016)
  • Author(s): Takizawa Y., Takano Y., Ohkouchi N., Yamamoto M., Chikaraishi Y.
  • Organizer: Goldschmidt 2016
  • Place of Presentation: Pacifico Yokohama（神奈川県横浜市）
  • Year and Date: 2016-06-26 – 2016-06-26
  • Int'l Joint Research
• [Presentation] Amino acid systematics using delta13C and delta15N in benthic archaeal methanotrophy at Black Sea (2016)
  • Author(s): Y. Takano, Y. Chikaraishi, N. O. Ogawa, M. Kruger, N. Ohkouchi
  • Organizer: International Conference on the Applications of Stable Isotope Techniques to Ecological Studies (IsoEcol 2016).
  • Place of Presentation: Univ Tokyo（東京都文京区）
  • Year and Date: 2016-04-07 – 2016-04-09
  • Int'l Joint Research
• [Remarks] 生物地球化学研究分野
  • URL: http://www.jamstec.go.jp/biogeochem/