Development of Small-molecule Fluorescent Probes Based on the Reactivity of Metal Complexes

2018 Fiscal Year Final Research Report

• Project/Area Number: 16H04124
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Inorganic chemistry
• Research Institution: Doshisha University
• Principal Investigator: Hitomi Yutaka 同志社大学, 理工学部, 教授 (20335186)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 過酸化水素 / 細胞 / 蛍光プローブ / 鉄錯体 / 反応 / 酸化

Outline of Final Research Achievements

We have developed a fluorescent probe that can rapidly visualize hydrogen peroxide released from cells as a signal. Fluorescent probes comprise complexes, profluorophores, and linkers linking them. In this study, the complex structure, profluorophore, and linker were improved. Hydrogen peroxide could be detected even in HeLa cells which do not overexpress the EGF receptor, when the most sensitive hydrogen peroxide fluorescent probe was used. In addition, it was shown that the emission of nitrogen monoxide could be achieved in the hypoxia region of cell spheroids by using MnNO complex. The mechanism by which the complex cleaves DNA by reductive oxygen activation was also elucidated.

Free Research Field

生物無機化学

Academic Significance and Societal Importance of the Research Achievements

生体で酸化反応をつかさどる酵素の機能を人工的な小分子で再現することに成功している。本研究ではその機能を大幅に向上させることに成功した。これらの成果は、微量な過酸化水素を検収することが必要なあらゆる場所で役立つ。