Protein synthesis using circular RNA

2018 Fiscal Year Final Research Report

• Project/Area Number: 16H04178
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Nagoya University
• Principal Investigator: Abe Hiroshi 名古屋大学, 理学研究科, 教授 (80415067)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 翻訳反応 / RNA

Outline of Final Research Achievements

Protein having a repetitive sequence is generated in a translation reaction on circular RNA template which contains a continuous open reading frame, because the ribosome performs translation rotating many times on the RNA. Optimization of the reaction condition in a E. coli cell-free translation system was performed. Development of efficient functional protein expression method in mammalian translation systems were carried out.

Free Research Field

核酸化学

Academic Significance and Societal Importance of the Research Achievements

近年、タンパク質を材料にした医薬品や生体材料の開発が進められている。連続的な読み枠を含む環状RNAを鋳型にしたタンパク質生合成反応を用いると、反復配列からなる長鎖タンパク質・ペプチドを簡便に調製できる。機能性タンパク質には反復配列を含むものが多く存在するため、これらの調製法として、本研究成果を用いることが出来る。