Development of fluorescent organic liquid nanoparticles and its application to cellular imaging

2018 Fiscal Year Final Research Report

• Project/Area Number: 16H04179
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Nagoya University
• Principal Investigator: TAKI Masaaysu 名古屋大学, 物質科学国際研究センター(WPI), 特任准教授 (70378850)
• Research Collaborator: Yamguchi Shigehiro
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 液体蛍光色素 / 有機蛍光ナノ粒子 / マルチカラー / 超耐光性蛍光色素 / 蛍光イメージング

Outline of Final Research Achievements

Organic fluorescent nanoparticles containing liquid fluorescent dye have been developed. We found that the particle size of fluorescent nanoparticles strongly depends on the viscosity of trialkylsilyl substituted pyrene, and the low viscosity liquid gave the smallest particle size. The fluidity of liquid pyrene is also maintained in the nanoparticle core, and the emission color was successfully tuned based on the new concept of "spontaneous incorporation of the dopant dyes into the nanoparticles". We also synthesized novel liquid fluorescent dyes with outstanding photostability, and examined the effect of the position of the silyl group on the physical properties. Furthermore, we prepared fluorescent nanoparticles containing the liquid dye and found that they are taken up inside the cell by the endocytosis mechanism.

Free Research Field

ケミカルバイオロジー

Academic Significance and Societal Importance of the Research Achievements

色素が液体であるという特徴を活かし，多様な発光色を示す有機蛍光ナノ粒子を「水溶液へのドーパントの添加」という極めて単純な方法で作り出す手法を発見したことは，今後の材料開発研究においても有用な情報である．また，液体蛍光ナノ粒子を用いて細胞の蛍光イメージングにも成功したことから，生命科学研究への応用も十分に期待できる．