Mechanism and evolution of 'non-self recognition by multiple factors' type self-incompatibility in rosaceous fruit trees

2020 Fiscal Year Final Research Report

• Project/Area Number: 16H04855
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Chiba University
• Principal Investigator: Sassa Hidenori 千葉大学, 大学院園芸学研究科, 教授 (50295507)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: 自家不和合性 / バラ科 / RNase / F-box / 雌ずい / 花粉

Outline of Final Research Achievements

Pyreae of Rosaceae (i.e., pear and apple) exhibit RNase-based self-incompatibility in which multiple pollen-part determinants SFBBs recognize non-self S-RNases, however, biochemical data supporting the model is not available partly because expression of recombinant SFBB protein is almost impossible.
In this study, we tested many protein expression systems and conditions, and established a SFBB protein expression system. Along with the newly developed tools for protein-protein interaction analysis, the SFBB expression will be useful for analysis of interaction between SFBB and S-RNase to gain insight into the molecular basis of self-incompatibility.

Free Research Field

植物遺伝育種学

Academic Significance and Societal Importance of the Research Achievements

バラ科の自家不和合性花粉側因子SFBBタンパク質の安定的な発現系はこれまでになく、自家不和合性研究の大きな障害になっていた。本研究で確立したSFBBタンパク質の発現系は、自家不和合性機構の解明にむけた重要な基盤となる。