Development of the selection method using the virus disease resistance gene in natural population.

2019 Fiscal Year Final Research Report

• Project/Area Number: 16H04970
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Sakamoto Takashi 東京海洋大学, 学術研究院, 教授 (40313390)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: ヒラメ / リンホシスチス / 耐病性 / ゲノム / 育種 / 遺伝子 / 養殖 / 選抜

Outline of Final Research Achievements

Japanese flounder is an economically important aquaculture species in Asian countries such as Japan, Korea and China. Lymphocystis disease (LD) is one of important disease and causes serious damage to Japanese flounder aquaculture industry. In this study, we investigated the virus disease resistant gene for LD and tried to develop the selection method using the sequences of the gene in natural population.
This research mainly accomplished three things as follows, 1) the development of the genotyping method with short-term rearing in wild juvenile fish, 2) performing the genome wide association study of the disease resistant trait on wild fish, 3) the finding of SNPs related with the disease resistant trait. These results would be very useful information to have the evidence of Lymphocystis disease resistant gene in Japanese flounder.

Free Research Field

水族分子遺伝育種学

Academic Significance and Societal Importance of the Research Achievements

本研究では、リンホシスチス病に対する耐病性責任遺伝子の同定を試みると共に、その責任遺伝子を用いた天然魚からの選抜技術の開発を行った。天然集団の中にリンホシスチス耐病性形質を保持する個体が少ないため、まだ統計的に有意な結果にはならなかったが、相関解析などの成果からこれまでの耐病性責任遺伝子候補がやはり有力な遺伝子候補であることが明らかになった。本研究成果により、天然魚において耐病性責任遺伝子を用いて直接的に耐病性個体を選抜し、新規耐病性系統を作れる可能性を示したと考えている。