Mechanisms for development of diseases by H. pylori virulence factor complex

2018 Fiscal Year Final Research Report

• Project/Area Number: 16H05191
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Oita University
• Principal Investigator: YAMAOKA YOSHIO 大分大学, 医学部, 教授 (00544248)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: ピロリ菌 / 外膜タンパク / タンパク質複合体 / 病原因子 / 炎症 / 抗体測定 / オルガノイド

Outline of Final Research Achievements

We succeeded to construct recombinant proteins of the whole length of OipA and CagA, the important virulence factors of Helicobacter pylori. Using mass spectrometry analyses, our results first suggested that OipA forms multimeric complex within the membrane region of H. pylori, or forms complex with other factors such as CagA. Furthermore, we established the most reliable system in Japan to measure anti-CagA antibody in serum samples. In addition, we succeeded to construct an ex-vivo model of human stomach, gastric epithelial organoid monolayers (gastric monolayers). Using this novel model, we expect to promote our research field on searching the interaction between H. pylori and gastric mucosa.

Free Research Field

消化器感染症

Academic Significance and Societal Importance of the Research Achievements

今回、我々が作成した血清中のCagA抗体を測定する系では、日本人由来のピロリ菌を材料としており、欧米のピロリ菌を用いている市販キットに比べて、正確性が高く、今後の汎用が期待される。また、今までは、ピロリ菌と胃粘膜との相互関係を調べるのに、胃がん細胞株が用いられていたが、2次元ガストロイドという新しい系を確立したことで、今後の基礎研究の飛躍的な発展が期待される。