Research on the mechanism of hepatocarcinogenesis by long-term replication of hepatitis virus

2018 Fiscal Year Final Research Report

• Project/Area Number: 16H05196
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Okayama University
• Principal Investigator: Kato Nobuyuki 岡山大学, 医歯薬学総合研究科, 教授 (40150883)
• Co-Investigator(Kenkyū-buntansha): 金 惠淑 岡山大学, 医歯薬学総合研究科, 准教授 (70314664) ; 佐藤 伸哉 岡山大学, 医歯薬学総合研究科, 助教 (80333558) ; 團迫 浩方 岡山大学, 医歯薬学総合研究科, 准教授 (80379841) ; 上田 優輝 岡山大学, 医歯薬学総合研究科, 助教 (90756074)
• Research Collaborator: SEJIMA Hiroe ; KOKU Irin ; ONOMURA Daichi ; HIRAMOTO Hiroki
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: C 型肝炎ウイルス / B型肝炎ウイルス / 長期HCV複製細胞 / 遺伝子発現変動 / HBV感受性 / Exosome

Outline of Final Research Achievements

We studied to clarify the relation of long-term replication of HBV or HCV and hepatocarcinogenesis, and then obtained the following results. (1) We clarified that VCX2 and AGR2 contributed to the expression control of the CPB2 gene whose expression level was remarkably decreased by the long-term HCV replication. (2) We successfully established two cloned cell lines exhibiting high HBV susceptibility by the subcloning of human immortalized hepatocyte NKNT-3 cells and human hepatoma Li23 cells. (3) We identified several genes whose expression levels significantly changed before and after HBV infection. (4) We developed the purification and quantitative methods for exosome produced from cultured cells. We demonstrated that the amount of exosome produced from HBV insusceptible cells was significantly higher than that of HBV susceptible cells.

Free Research Field

ウイルス学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、HCVやHBV感染により高頻度に生じる肝癌の発症を未然に防ぐ方法の開発に資する知見となるので、学術的意義があるばかりでなく、難治癌の１つで我が国に多い肝癌肝癌患者に対する社会的意義も大きい。今後も継続してこの関連の研究を展開していく必要性を示す研究成果になったことは、この研究課題の大きさを物語っていると言える。