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2018 Fiscal Year Final Research Report

Analysis of the mechanisms underlying clonal hematopoiesis in patients with acquired aplastic anemia

Research Project

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Project/Area Number 16H05335
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionKanazawa University

Principal Investigator

Nakao Shinji  金沢大学, 医学系, 教授 (70217660)

Co-Investigator(Renkei-kenkyūsha) Yoshida Yoshinori  京都大学, iPS細胞研究所, 准教授 (20447965)
Kishi Hiroyuki  富山大学, 大学院医学薬学研究部, 教授 (60186210)
Hosomichi Kazuyoshi  金沢大学, 医学系, 准教授 (50420948)
Project Period (FY) 2016-04-01 – 2019-03-31
Keywords再生不良性貧血 / クローン性造血 / HLA / 細胞傷害性T細胞 / 自己抗原
Outline of Final Research Achievements

Acquired aplastic anemia (AA) is a kind of autoimmune disease that is induced by the cytotoxic T lymphocyte attack against hematopoietic stem progenitor cells (HSPCs) although its exact pathogenesis remains unclear. In some patients with AA, a limited number of HSPCs clonally proliferate and support hematopoiesis without showing a tendency toward the development of hematologic malignancies. We investigated the mechanisms underlying such clonal hematopoiesis in AA patients, and found that abnormal leukocytes that lack HLA class I alleles due to loss-of-function mutations in limited HLA class I genes such as B*40:02、A*02:06 and B*54:01 are often detectable. Of particular interest, 33% of the patients shared a common nonsense mutation across different HLA alleles, that also causes the loss of corresponding HLA alleles and is therefore thought to serves as a reliable marker of immune pathophysiology of AA.

Free Research Field

血液内科学

Academic Significance and Societal Importance of the Research Achievements

再不貧では、第6染色体短腕のloss of heterozygosity (6pLOH)によってHLAクラスIアレルを欠失した白血球が検出されることが我々の研究によって分かっていたが、欠失するハプロタイプ中のどのHLAが自己抗原を提示しているかは不明であった。本研究により、再不貧はHLA-B*40:02、B*54:01などの限られたHLAアレルが造血幹細胞抗原をTリンパ球に提示することによって起こる自己免疫疾患であることが明らかになった。これらの特定のアレルを持つ例のTリンパ球を調べれば、自己抗原が明らかになる可能性が高い。また、HLAの共通変異の検出は免疫病態の証明に非常に有用である。

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Published: 2020-03-30  

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