Latitudinal pattern of methanotrophic food webs driving carbon and nitrogen cycling in lakes

2018 Fiscal Year Annual Research Report

• Project/Area Number: 16H05774
• Research Institution: Research Institute for Humanity and Nature
• Principal Investigator: 奥田 昇 総合地球環境学研究所, 研究部, 准教授 (30380281)
• Co-Investigator(Kenkyū-buntansha): 伊藤 雅之 兵庫県立大学, 環境人間学部, 准教授 (70456820) ; 藤林 恵 秋田県立大学, 生物資源科学部, 助教 (70552397)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: メタン栄養食物網 / メタン酸化細菌 / メタン / 炭素・窒素循環 / NC10 / 部分循環湖 / 一回循環湖 / 酸化還元環境

Outline of Annual Research Achievements

異なる気候帯に属する湖沼のメタン酸化細菌（MOB）叢およびメタン栄養食物網の気候応答メカニズムを理解するために、熱帯のセブンレイク、亜熱帯の翡翠水庫、温帯の琵琶湖において比較観測調査を実施した。
1-a) セブンレイク：深度の異なる7つの火山湖沼群で観測調査を行ったところ、水深60m以深の湖は部分循環湖に分類され、38m以浅の湖では、雨季から乾季の移行期に吹く冷温な北東風によって全循環することが明らかとなった。部分循環湖では、強還元な水温躍層下で1mM/L以上の高濃度メタンの貯留が周年観察された。
1-b) 翡翠水庫：長期観測調査の結果、暖冬年には循環不全が起こり、続く夏季成層期に還元化した深底層でメタン貯留が増大した。他方、冷冬年には全循環が起こり、深底層のメタン貯留が消失した。2018年から続く暖冬の影響により、2019年には、深底層で60μM/L以上のメタン濃度が観測された。
1-c) 琵琶湖：冬季に全循環が起こる一回循環湖と分類されるが、2018年以降の暖冬傾向により、循環不全と深底層の貧酸素化が観察された。メタン濃度は全層を通じて極めて低く、近底層にメタン亜極大が観察されるものの最大でも0.2μM/L以下だった。
2) MOB叢・メタン栄養食物網：いずれの湖沼でも、MOBの主要分類群であるType I・Type II、さらに、嫌気的MOBであるNC10が観察されたが、その密度や深度分布は観測年や季節によって湖沼間で大きく変動した。琵琶湖と翡翠水庫のMOB叢は分子系統的に近縁関係にあったが、セブンレイクのType I・Type IIの出現分類群は前2者と大きく異なり、さらに、新奇なメチル栄養細菌が高頻度で観察された。MOBから動物プランクトンに栄養転送される脂肪酸の割合は、いずれの湖沼においても数%程度と低かったが、還元的なセブンレイクではNC10由来の脂肪酸が検出された。

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] サント・トーマス大学(フィリピン)
  • Country Name: PHILIPPINES
  • Counterpart Institution: サント・トーマス大学
• [Int'l Joint Research] 中央研究院/国立臺灣大学(台湾)
  • Country Name: その他の国・地域
  • Counterpart Institution: 中央研究院/国立臺灣大学
• [Journal Article] Nutrient loadings and deforestation decrease benthic macroinvertebrate diversity in an urbanised tropical stream system (2020)
  • Author(s): Peralta Elfritzson M., Batucan Leocris S., De Jesus Irisse Bianca B., Trino Ellis Mika C., Uehara Yoshitoshi, Ishida Takuya, Kobayashi Yuki, Ko Chia-Ying, Iwata Tomoya, Borja Adelina S., Briones Jonathan Carlo A., Papa Rey Donne S., Magbanua Francis S., Okuda Noboru
  • Journal Title: Limnologica Volume: 80 Pages: 125744～125744
  • DOI: https://doi.org/10.1016/j.limno.2019.125744
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Small Maar Lakes of Luzon Island, Philippines: Their Limnological Status and Implications for the Management of Tropical Lakes . A Review (2019)
  • Author(s): Mendoza, M. U., Briones, J. C. A., Itoh, M., Padilla, K. S. A. R., Aguilar, J. I., Okuda, N., Papa, R. D. S.
  • Journal Title: Philippine Journal of Science Volume: 148 Pages: 559～572
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Stream benthic macroinvertebrate assemblages reveal the importance of a recently established freshwater protected area in a tropical watershed (2019)
  • Author(s): Peralta, E. M., A. E. Belen, G. R. Buenaventura, F. G. G. Cantre, K. G. R. Espiritu, J. N. A. De Vera, C. P. Perez, A. K. V. Tan, I. B. B. De Jesus, P. Palomares, J. C. A. Briones, T. Ikeya, F. S. Magbanua, R. D. S. Papa, N. Okuda
  • Journal Title: Pacific Science Volume: 73 Pages: 305～320
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The biogeochemical cycling of methane (CH4) in lakes Calibato, Pandin and Yambo: Implications on the lakes’ carrying capacity (2019)
  • Author(s): Mendoza, M. U., J. I. Aguilar, K. S. A. R. Padilla, M. Itoh, N. Okuda, R. D. S. Papa
  • Journal Title: Transactions of the National Academy of Science & Technology Philippines Volume: 41 Pages: 139
  • Int'l Joint Research
• [Journal Article] Disentangling multiple stressors and highlighting the importance of freshwater protected area in highly urbanized watersheds in the Philippines (2019)
  • Author(s): Peralta E., F. S. Magbanua, J. C. A. Briones, N. Okuda, R. D. S. Papa
  • Journal Title: Transactions of the National Academy of Science & Technology Philippines Volume: 41 Pages: 146
  • Int'l Joint Research
• [Presentation] 湖からの拡散による温室効果ガスの放出のモデル検証 (2020)
  • Author(s): 田岡作, 岩田拓記, 宮原裕一, 平田竜一, 高橋善幸, 伊藤雅之
  • Organizer: 日本農業気象学会
• [Presentation] Methanotrophic food webs in tropical lakes (2020)
  • Author(s): Okuda, N., M. U. Mendoza, J. I. Aguilar, K. S. A. R. Padilla, J. C. A. Briones, R. D. S. Papa, M. Ito, M. Fujibayashi, T.-H. Tu, L.-H. Lin, P.-L. Wang, Y. Kobayashi, E. Austria & F.-K. Shiah
  • Organizer: JpGU-AGU Joint Meeting
  • Int'l Joint Research
• [Presentation] Simulation of methane dynamics in a mid-latitude eutrophic lake with constraints using incubation data (2019)
  • Author(s): Iwata, H., Nakano, W., Stepanenko, V., Kobayashi, D., Taoka, T., Itoh, M., Miyabara, Y., Hirata, R., Takahashi, Y.
  • Organizer: AsiaFlux
  • Int'l Joint Research
• [Presentation] Methanotrophic food webs in tropical lakes: a preliminary report (2019)
  • Author(s): Okuda, N., Milette, M., Aguilar, J., Padilla, K., Briones, J., Papa, R., Itoh, M.., Fujibayashi, M., Tu, T-H., Lin, L-H., Wang, P-L., Kobayashi, Y., Austria, E., Shiah, F-K.
  • Organizer: 日本陸水学会
• [Presentation] Watershed governance: a case in the Lake Biwa Watershed (2019)
  • Author(s): Okuda, N
  • Organizer: RIHN International Symposium: Fair use of multiple resources in cross-scale context
  • Int'l Joint Research / Invited
• [Presentation] Vertical profiles of methane-oxidizing bacteria in selected tropical maar lakes of San Pablo, Laguna, Philippines (2019)
  • Author(s): Padilla KSAR, Mendoza MU, Aguilar JI, Austria ES, Lin LH, Tu TH, Shiah FK, Papa RDS, Okuda N
  • Organizer: Graduate Scholars’ Conference
• [Presentation] Exploring methane (CH4) storage in small tropical lakes (2019)
  • Author(s): Mendoza MU, Aguilar JI, Padilla KSAR, Itoh M, Okuda N, Papa RDS
  • Organizer: Graduate Scholars’ Conference
• [Presentation] Long-term limnological monitoring revealed the spatio-temporal patterns of the Seven Maar Lakes (SML’s) of Laguna (2019)
  • Author(s): Aguilar JI, Mendoza MU, Padilla KSAR, Papa RDS, Okuda N
  • Organizer: Graduate Scholars’ Conference
• [Presentation] Multiple stressors and protection efforts in highly urbanized watersheds in the Philippines (2019)
  • Author(s): Peralta, E. M., L. S. Batucan Jr., A. E. Belen, I. B. B. De Jesus, T. Ishida, C.-Y. Ko, Y. Kobayashi, T. Ikeya, Y. Uehara, T. Iwata, A. S. Borja, J. C. A. Briones, F. S. Magbanua, R. D. S. Papa, N. Okuda
  • Organizer: JpGU-AGU Joint Meeting
  • Int'l Joint Research
• [Presentation] Benthic macroinvertebrate assemblages and water quality reflect the impacts of land use and land cover in Marikina Watershed, Philippines (2019)
  • Author(s): Gregorio, J. A. N., E. M. Peralta, I. B. B. De Jesus, T. Ikeya, J. C. A. Briones, R. D. S. Papa, F. S. Magbanua, N. Okuda
  • Organizer: JpGU-AGU Joint Meeting
  • Int'l Joint Research
• [Presentation] 琵琶湖の流域と地域の問題を解決する取り組み (2019)
  • Author(s): 奥田昇
  • Organizer: 八郎潟・八郎湖学研究会
  • Invited
• [Presentation] Carbon and nitrogen stable isotopes reveal impacts of human activities on community assemblages and food web structures in Laguna de Bay and Marikina Watershed (2018)
  • Author(s): Peralta EM, Magbanua FS, Briones JCA, Okuda N, Papa RDS
  • Organizer: Philippine Symposium on Freshwater Biodiversity and Ecosystems
  • Int'l Joint Research
• [Presentation] Vertical distribution of methane-oxidizing bacteria in tropical Maar Lakes Yambo, Pandin, and Calibato in San Pablo, Laguna (2018)
  • Author(s): Padilla KSAR, Aguilar JI, Mendoza MU, Okuda N, Papa RDS
  • Organizer: Philippine Symposium on Freshwater Biodiversity and Ecosystems
  • Int'l Joint Research
• [Presentation] What has changed in the Twin Lakes of San Pablo, Laguna, Philippines (2008 vs 2018)? (2018)
  • Author(s): Mendoza MU, Tordesillas DT, Padilla KSAR, Aguilar JI, Okuda N, Papa RDS
  • Organizer: Philippine Symposium on Freshwater Biodiversity and Ecosystems
  • Int'l Joint Research
• [Presentation] The adaptive watershed governance: science for society (2018)
  • Author(s): Okuda, N.
  • Organizer: Philippine Symposium on Freshwater Biodiversity and Ecosystems
  • Int'l Joint Research / Invited
• [Presentation] Stream benthic macroinvertebrate assemblages reflect anthropogenic impacts and protection status of in the two watersheds of Laguna de Bay, Philippines (2018)
  • Author(s): Peralta, E. M., F. S. Magbanua, J. C. A. Briones, N. Okuda, R. D. S. Papa
  • Organizer: Annual Convention of the Federation of Institutions for Marine and Freshwater Sciences (FIMFS)
• [Presentation] Monsoon-driven variations in the vertical profile of the Seven Maar Lakes of San Pablo, Laguna: Diving deeper in the limnology of tropical maar lakes (2018)
  • Author(s): Aguilar, J. F., P. Cootauco, J. A. T. DE Guzman, P. A. Mauricio, C. Remperas, N. Okuda, R. D. S. Papa R
  • Organizer: Annual Convention of the Federation of Institutions for Marine and Freshwater Sciences (FIMFS)
• [Presentation] What can we learn from determining methane concentrations in freshwater lake ecosystems? (2018)
  • Author(s): Mendoza, M. U., K. S. R. Padilla, J. Aguilar, M. Itoh, N. Okuda, R. D. S. Papa
  • Organizer: Annual Convention of the Federation of Institutions for Marine and Freshwater Sciences (FIMFS)
• [Presentation] Watershed governance to enhance social-ecological health (2018)
  • Author(s): Okuda, N.
  • Organizer: World Lake Conference
  • Int'l Joint Research / Invited
• [Presentation] The adaptive watershed governance: linking local and global scales (2018)
  • Author(s): Okuda, N.
  • Organizer: International Symposium of Future Earth
  • Int'l Joint Research / Invited
• [Presentation] Littoral food web analysis: Stable isotopes reveal anthropogenic impacts on Oreochromis niloticus and its food sources in Laguna de Bay (2018)
  • Author(s): Peralta, E. M., C. B. Bacinillo, J. C. A. Balani, S. E. Basmala, E. J. S. S. Calalin, M. A. G. Calleja, J. B. C. Go, M. T. S. Gosiengfiao, F. A. T. Valdecanas, J. N. Gregorio, N. Mendoza, T. Ishida, F. S. Magbanua, J. C. A. Briones, R. D. S. Papa, N. Okuda
  • Organizer: Fisheries Science Conference
• [Presentation] Are the ‘Twin Lakes’of San Pablo ‘identical’? (2018)
  • Author(s): Aguilar, J. F., K. S. A. Padilla, M. U. Mendoza, N. Okuda, R. D. S. Papa
  • Organizer: Congress of International Society of Limnology
  • Int'l Joint Research
• [Presentation] Monsoon-based variations in the vertical profile of selected limnological parameters in in the Seven Maar Lakes (SML’s) of Laguna (Luzon Is., Philippines): A unique insight into the limnology of tropical maar lakes (2018)
  • Author(s): Papa R. D. S., J. F. I. Aguilar, P. A. B. Cootauco, J. A. P. De Guzman, P. A. C. Mauricio, C. T. A. Remperas, N. Okuda
  • Organizer: Congress of International Society of Limnology
  • Int'l Joint Research
• [Presentation] Zooplankton Community Structure in the Littoral Zones of Laguna de Bay reflects Land Cover and Nutrient Loading in near-shore areas (2018)
  • Author(s): Guinto, M. M. V., H. A. Guerrero, J. C. A. Briones, R. D. S. Papa, N. Okuda
  • Organizer: JpGU-AGU Joint Meeting
  • Int'l Joint Research
• [Presentation] Stable Isotopes Reveal Anthropogenic Impacts on the Littoral Food Webs of Laguna de Bay, Philippines (2018)
  • Author(s): Peralta, E. M., C. Bacinillo, J. C. Balani, S. Basmala, E. J. S. Calalin, M. A. Calleja, J. B. Go, M. T. Gosiengfiao, F. A. Valdecanas, J.-A. Gregorio, N. Mendoza, T. Ishida, F. Magbanua, J. C. Briones, R. D. Papa, N. Okuda
  • Organizer: JpGU-AGU Joint Meeting
  • Int'l Joint Research
• [Presentation] フィリピン・ラグナ湖流域の栄養循環：琵琶湖流域との比較 (2018)
  • Author(s): 奥田昇, Irisse Bianca De Jesus, Osbert Leo A. Privaldos, 岩田智也, 上原佳敏, 石田卓也, 淺野悟史, 尾坂健一, 藤吉麗, 由水千景, 陀安一郎, Jonathan Carlo A. Briones, Francis S. Magbanua, Maria Pythias B. Espino, Adelina C. Santos-Borja, Rey Donne S. Papa
  • Organizer: 水文科学会学術大会
  • Invited
• [Presentation] 琵琶湖流域の栄養循環：統合的理解を目指して (2018)
  • Author(s): 奥田昇, 小野寺真一, 伴修平
  • Organizer: 日本陸水学会
• [Funded Workshop] nternational Workshop: Latitudinal pattern of methanotrophic food webs in lakes (2018)