2016 Fiscal Year Annual Research Report
Investigating the resilience of Japan's cool climate forests to past and ongoing climate change
| Project/Area Number |
16H06197
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
Worth James 国立研究開発法人森林総合研究所, 樹木分子遺伝研究領域, 主任研究員 (30770771)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | Fagus crenata / Japanese Tsuga / NGS / Range-edge populations / Adaptive genes |
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| Outline of Annual Research Achievements |
In the first year of the study I have obtained the whole chloroplast genome from individuals from 13 populations of Fagus crenata in lowland Kanto and 7 from southern Kyushu. This has resulted in the discovery of tens of DNA polymorphisms within these two regions including 25 microsatellite regions.
For Tsuga diversifolia I have collected 32 populations from across the species range. In order to assess whether Tsuga diversifolia hybridizes with Tsuga sieboldii I have collected 30 populations of this species, including locations where both species come into close proximity. I have developed 15 nuclear, 16 chloroplast and 24 mitochondrial SSR markers for both species.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The studies of both Fagus crenata and Japanese Tsuga are both progressing well with molecular markers all developed and sampling almost completed. However, I was unable to achieve my goal of utilizing the developed markers to screen all samples.
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| Strategy for Future Research Activity |
For the second year of the study I will use the markers developed in the first year to screen all samples of both Fagus crenata and Japanese Tsuga. This will reveal the genetic divergence and diversity of warm edge populations.
I also aim to complete the sampling of Fagus crenata at 4 locations where the species occurs across an elevational range of 700 to 1000 m over short geographic distances, with two such sites in both the Japan Sea and Pacific Ocean side of Japan, respectively. These will be then genotyped using RAD-seq in order to detect DNA polymorphisms under probable selection for temperature.
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