2017 Fiscal Year Annual Research Report
Investigating the resilience of Japan's cool climate forests to past and ongoing climate change
| Project/Area Number |
16H06197
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| Research Institution | Forest Research and Management Organization |
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Principal Investigator |
Worth James 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (30770771)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | Tsuga diversifolia / Tsuga sieboldii / Fagus crenata / NGS / SSR markers / population genetics |
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| Outline of Annual Research Achievements |
I designed nuclear and chloroplast SSR markers for Japanese Tsuga species. Using these I have genotyped 35 populations for each of Tsuga sieboldii and T. diversifolia from across their ranges. No evidence of hybridisation was observed between the species. There was significant genetic structuring of genetic diversity in both species.
For Fagus crenata I have assembled the whole chloroplast genome. A phylogenetic tree based on whole chloroplast genomes of the major haplotypes in Fagus crenata showed two diverged clades in western and eastern Japan. Unexpectedly the Chinese F. engeleriana found to be nested within the western F. crenata clade.
I have also obtained RAD-seq data from 354 samples of Fagus crenata sampled from 4 altitudinal clines.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
I have been able to achieve my goals for the 2nd year of this project.
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| Strategy for Future Research Activity |
I will publish a paper on the genetic diversity and structure of Japanese Tsuga species based on nuclear and chloroplast SSR markers.
I also plan to complete the analysis of RAD-seq data to identify candidate SNPs under selection for temperature across 4 independent altitudinal clines and publish a paper. This will be aided by the soon to released whole genome sequence of F. crenata.
Lastly, I plan to complete sampling of Thuja sieboldii and develop nuclear SSR markers to discover how long small isolated populations in western Japan at the warm edge of the species range have persisted.
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