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2018 Fiscal Year Annual Research Report

Investigating the resilience of Japan's cool climate forests to past and ongoing climate change

Research Project

Project/Area Number 16H06197
Research InstitutionForest Research and Management Organization

Principal Investigator

Worth James  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (30770771)

Project Period (FY) 2016-04-01 – 2019-03-31
KeywordsTsuga diversifolia / Tsuga sieboldii / Fagus crenata / NGS / SSR markers / population genetics
Outline of Annual Research Achievements

Nuclear and chloroplast SSR markers for Japanese Tsuga species were designed using NGS. Using these markers significant genetic structure across the species ranges was found with both northern and southernmost populations the most diverged. Genetic diversity is correlated with past logging in the temperate forest species, T. sieboldii, but not in the subalpine T. diversifolia where human impacts have been less. Although not identified using SSR markers RAD-seq data identified low levels of hybridisation between the two species.
In Fagus crenata, whole chloroplast genome sequencing revealed that there are two strongly diverged clades in Japan, one in western Japan that is closest to the chinese species F. engleriana and the other in eastern Japan.

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

  • Research Products

    (4 results)

All 2018

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results) Presentation (2 results) (of which Invited: 2 results)

  • [Journal Article] 四国・石鎚山系においてコメツガ南限集団を確認する(Confirmation of the most southerly occurrence of Tsuga diversifolia at Mt. Ishizuchi,Shikoku,Japan,using genetic data)2018

    • Author(s)
      James R. P. Worth(ワース ジェームズ)、TSUYAMA Ikutaro(津山幾太郎)、KIKUCHI Satoshi(菊地賢)、AIZAWA Mineaki(逢沢峰明・宇都宮大学)
    • Journal Title

      植物地理・分類研究

      Volume: 66 Pages: 185-192

    • DOI

      DOI:10.18942/chiribunrui.0662-14

    • Peer Reviewed
  • [Journal Article] Whole chloroplast genome sequences of the Japanese hemlocks,Tsuga diversifolia and T. sieboldii,and development of chloroplast microsatellite markers applicable to East Asian Tsuga(日本のツガ、コメツガの全葉緑体ゲノムシーケンスと東アジアのツガに応用可能な葉緑体マイクロサテライトマーカーの開発)2018

    • Author(s)
      Luxian Liu(Zhejiang University)、Pan Li(Zhejiang University)、H.W Zhang(Zhejiang Qingliangfeng National Natural Reserve)、James R. P. Worth(ワース ジェームズ)
    • Journal Title

      Journal of Forest Research

      Volume: 23 Pages: 318-323

    • DOI

      doi.org/10.1080/13416979.2018.1512395

    • Peer Reviewed / Int'l Joint Research
  • [Presentation] When species collide: consequences of range-overlap of distantly related island endemic conifers2018

    • Author(s)
      James Worth
    • Organizer
      第129回日本森林学会大会
    • Invited
  • [Presentation] Speciation of Tsuga in Ulleung Island based on nuclear and chloroplast SSRs2018

    • Author(s)
      James Worth
    • Organizer
      The potential of genomics in plant systematics
    • Invited

URL: 

Published: 2019-12-27  

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