2017 Fiscal Year Final Research Report
Elucidation of the mechanism of radiation-induced leukemia on human hematopoietic stem cells
| Project/Area Number |
16H06611
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Research Collaborator |
KASHIWAKURA Ikuo
EBINA Satoko
HIROUCHI Tokuhisa
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Keywords | ヒト造血幹細胞 / 電離放射線 / 遺伝子発現解析 / 白血病 |
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| Outline of Final Research Achievements |
To clarify the nature of genes and related signaling pathways that contribute to the radiation-related leukemia on human hematopoietic stem cells (HSCs), we analyzed the gene expression profiles and its related signaling pathways detected in HSCs irradiated with 2 Gy X-rays before culture with or without an optimal combination of hematopoietic cytokines. In the absence of hematopoietic cytokines, expression control pathways related to carcinogenesis such as RB/E2F, Est and p53, and carcinogenesis such as C/EBP were enhanced after X-rays irradiation. On the other hand, culture with cytokine combination (recombinant human thrombopoietin plus interleukin-3 plus stem cell factor) that exerts radiation protection effect showed a specific enhancement of CD44 signaling pathways.It is suggested the importance of CD44 signaling pathway to the radiation response of human HSCs.
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| Free Research Field |
放射線科学
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