2017 Fiscal Year Final Research Report
Construction of tethered lipid bilayer with domain and its application to membrane protein research
| Project/Area Number |
16H06667
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical chemistry
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Keywords | 脂質二重膜 / 原子間力顕微鏡 / 蛍光顕微鏡 / 膜タンパク質 / 自己組織化単分子膜 / マイカ |
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| Outline of Final Research Achievements |
The artificial lipid membrane (SLB) formed on the substrate is useful for understanding the molecular structure of membrane proteins from its two-dimensional nature. On the other hand, structure deformation and lack of orientation due to contact with the substrate are obstacles in membrane protein research. It is necessary to reduce contact with the substrate and immobilize the membrane protein in a specific orientation. In this study, self-assembled monolayer (SAM) binding to a specific site of membrane protein was formed on flat mica, and SLB was formed thereon. It was confirmed that the orientation was aligned from the measurement of the molecular sizes of the membrane proteins with different extracellular domains on the top and bottom. Inactivation of membrane proteins can be reduced and structural analysis of membrane proteins with specific orientation has become possible.
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| Free Research Field |
物理化学
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