2017 Fiscal Year Final Research Report
Analysis of circulating tumor DNA of head and neck cancer using dropet digital PCR
Project/Area Number |
16H06759
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Otorhinolaryngology
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Research Institution | The University of Tokyo |
Principal Investigator |
Akashi Ken 東京大学, 医学部附属病院, 助教 (90779331)
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Research Collaborator |
YAMASOBA Tatsuya
ANDO Mizuo
YOSHIDA Masafumi
SAITO Yuki
FUKUOKA Osamu
MURAKAMI Yoshinori
|
Project Period (FY) |
2016-08-26 – 2018-03-31
|
Keywords | 血中循環腫瘍DNA / デジタルPCR / 頭頸部癌 / EBV関連上咽頭癌 / HPV関連中咽頭癌 |
Outline of Final Research Achievements |
The objective of this study was to examine whether ctDNA of virus-related head and neck cancer could be detected using droplet digital polymerase chain reaction (PCR), which enables quantification of nucleic acids with a higher accuracy, sensitivity, and precision than traditional real-time PCR. We collected plasma from blood samples of 4 patients with EBV-related nasopharyngeal cancer and 18 patients with HPV-related oropharyngeal cancer. We extracted cell-free DNA from the plasma and detected ctDNA using droplet digital PCR targeting base sequences of EBV-DNA and HPV-DNA integrated into the DNA of cancer cells. We successfully detected ctDNA in the blood of 3 patients (75%) with EBV-related nasopharyngeal cancer and 10 patients (56%) with HPV-related oropharyngeal cancer.
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Free Research Field |
頭頸部外科
|