Analysis of cell cycle related signals for inhibition of histone acetylation in pancreatic cancer cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H06773
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: ONO Hiroaki 東京医科歯科大学, 医学部附属病院, 助教 (60466901)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: 膵臓癌 / 細胞周期 / 抗がん剤 / 腫瘍外科 / 分子細胞学

Outline of Final Research Achievements

Histone Acetylation plays a significant role in DNA replication and regulation of cell cycle. We tried to investigate its relationship between Histone acetylation and cell cycle especially in pancreatic cancer. Our study has shown evidence that Histone acetylation such as H3K9Ac and H3K27Ac is activated in pancreatic cancer cell lines. Furthermore, C646, which is a biological inhibitor of Histone acetylation, inhibited cell cycle and induced cell apoptosis of pancreatic cancer cell lines as well. Cell cycle related genes of cyclin B1 and CDC2 were also inhibited by C646 treatment. These data suggested that down-regulation of Histone acetylation induced G2/M cell cycle arrest through inhibition of cell cycle related genes at transcriptional level.

Free Research Field

消化器外科学