Identification and validation of the angiogenic adipose-derived stem cells among patients with cardiovasciular diseases

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H06828
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Cardiovascular medicine
• Research Institution: Kanazawa University
• Principal Investigator: Oto Inoue 金沢大学, 附属病院, 医員 (00781147)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: 脂肪組織由来間葉系幹細胞 / 血管新生

Outline of Final Research Achievements

Adipose-derived stem cells (ADSCs) have been emerged as an attractive option for regenerative therapy. ADSCs are composed of various subsets and the CD271+ subset is reported to be more primitive and proliferative. ADSCs were isolated from subcutaneous adipose tissue of 20 patients undergoing elective cardiac surgery. The percentages of CD271+ subset in CD45-CD34+ ADSCs were measured by flowcytometer. According to the CD271 frequency, ADSCs samples were divided into two groups (“high” and “low” groups). Nude mice were subjected to hindlimb ischemia and 1 million of ADSCs) were injected into the ischemic muscles. At day 14, neovascularization was evaluated by laser Doppler analysis and histological analysis using fluorescent-labeled lectin perfusion in duplicate. Intramuscular injection of ADSCs of the “high” group demonstrated enhanced neovascularization compared to the “low” group in laser Doppler analysis and also in histological analysis.

Free Research Field

再生医療