Development of asymmetric oxidazing enzyme acting on prochiral diols and synthesis of lead compounds for drug

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H06843
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Gifu University
• Principal Investigator: KIKUKAWA Hiroshi 岐阜大学, 工学部, 助教 (80758805)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: プロキラルジオール / 不斉酸化 / ヒドロキシアルカン酸 / アルコール脱水素酵素 / プロテオーム解析

Outline of Final Research Achievements

In the first year, we found microbes A and B asymmetrically oxidizing 2,2-diethyl-1,3-propanediol. As a result of optical purity analysis, strain A synthesized (R)-hydroxypropanoate at 65%ee, and strain B synthesized (S)-hydroxypropanoate at 33%ee.

In the second year, we aimed to isolate and characterize the genes coding asymmetrically oxidizing enzyme using heterologous expression system. Several candidate genes were selected through both proteome and whole-genome analysis, and were heterologously expressed. As a result of optical purity analysis, three recombinants exhibited (R)-selectivity. One of the recombinant expressing an alcohol dehydrogenase synthesized (R)-hydroxypropanoate at 90%ee.

Free Research Field

応用微生物学