Development of treatment methods of tendon diseases using genetically modified mice

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H06845
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Gifu University
• Principal Investigator: Komura Shingo 岐阜大学, 医学部附属病院, 助教 (30456511)
• Research Collaborator: Yamada Yasuhiro
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: 腱細胞 / 腱鞘滑膜細胞 / 関節滑膜細胞 / Scx / Tppp3

Outline of Final Research Achievements

Scleraxis (Scx) is tendon specific marker, and tubulin polymerization-promoting protein family member 3 (Tppp3）is tendon sheath and synovial joint specific marker. We generated Scx and Tppp3-reporter mouse; Scx-EGFP and Tppp3-EGFP, and tamoxifen-inducible Cre mouse; Scx-CreERT2 and Tppp3-CreERT2. Scx-EGFP and Scx-CreERT2/R26-LacZ mice specifically labeled tendons and ligaments in vivo. While Tppp3-EGFP and Tppp3-CreERT2/R26-LacZ mice labeled not only tendon sheath and synovium in the joints but also other tissue and organs such as bronchial epithelium, nervous system, skin.
To investigate whether tendon sheath cells and synovial cells contribute to tendon regeneration, we generated patellar tendon injury model utilizing Tppp3-CreERT2/R26-LacZ mice. 1 week after tendon injury, the gap between ruptured tendons was filled with X-gal negative fibroblastic cells, indicating that tendon sheath cells and synovial cells around patellar tendon could not contribute to tendon regeneration.

Free Research Field

整形外科学