Functional analysis of the novel apoptosis suppressor Apsup from the baculovirus Lymantria dispar multiple nucleopolyhedrovirus

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H06866
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Insect science
• Research Institution: Nagoya University
• Principal Investigator: Yamada Hayato 名古屋大学, 生命農学研究科, 助教 (70778258)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: バキュロウイルス / Apsup / アポトーシス / マイマイガ / 抗ウイルス応答 / 昆虫培養細胞 / 核多角体病ウイルス

Outline of Final Research Achievements

We have previously identified a novel apoptosis suppressor Apsup from the baculovirus Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV). Apsup suppresses apoptosis by preventing proteolytic processing of L. dispar initiator caspase Ld-Dronc. Here, we performed mutation analyses of Apsup to determine functional domains in Apsup. Mutation analyses indicated that the N-terminal region in Apsup required for interacting with Ld-Dronc, and the C-terminal region in Apsup required for preventing the proteolytic processing of Ld-Dronc. Our findings provide clues to understand the mechanisms by which Apsup suppresses apoptosis.

Free Research Field

昆虫科学